Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal spermatozoa

Theriogenology
J Santiago-MorenoA López-Sebastián

Abstract

Tris-egg yolk based diluents provide adequate cryoprotection for the sperm of most wild species in which they have been tested. The objective of the current study was to evaluate various Tris-based diluents containing different concentrations of egg yolk, for the fertilizing ability of epididymal spermatozoa of the Spanish ibex (Capra pyrenaica) after freezing and thawing. For this purpose, we used heterologous in vivo fertilization by intrauterine insemination of domestic goats (Capra hircus). In Experiment 1, a Tris-citric acid-glucose (TCG) diluent containing 6% (v/v) egg yolk and a TCG extender containing 20% egg yolk were compared. In Experiment 2, a TCG-6% egg yolk extender was compared with Triladyl-20% egg yolk. Diluted samples were cooled slowly to 5 degrees C over 1 h and equilibrated at that temperature for 2 h. At that point, aliquots of samples were loaded into 0.25 ml straws, and frozen in nitrogen vapor for 10 min. The fertility of spermatozoa frozen in TCG-6% egg yolk was higher (P<0.05) than for those extended with TCG-20% egg yolk, and tended to be higher than for those frozen with Triladyl-20% egg yolk. From the results of this study, the use of Tris-based extenders containing low concentrations of egg yolk (...Continue Reading

References

May 1, 1976·Journal of Reproduction and Fertility·P F Watson
Feb 1, 1974·Theriogenology·V G Pursel, L A Johnson
Aug 5, 2000·Animal Reproduction Science·W V Holt
Jan 5, 2005·Biochimica Et Biophysica Acta·Barbara SiasFrédéric Carrière
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Lamia AmiratJean Luc Courtens
Nov 1, 1990·Theriogenology·M S Chauhan, S R Anand

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Citations

Mar 25, 2008·Archives of Andrology·Julián Santiago-MorenoAntonio López-Sebastián
Sep 26, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Marisa BezjianKatherine A Beltaire

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