Effect of egg yolk plasma on dog sperm cryopreservation

Andrologia
C D CorciniA S Varela Junior

Abstract

This study evaluated the quality of frozen-thawed dog spermatozoon after the inclusion of egg yolk plasma (EYP) instead of whole egg yolk (EY) in the cryopreservation extender and after distinct periods of exposure to EYP. Seven mongrel dogs were used as sperm donors, and EYP was obtained by centrifugation. In Experiment 1, post-thawing sperm motility (MOT) and integrity of membrane (INT) and acrosome (ACR) were superior for spermatozoon extended with 20% EYP T2 than with 20% EY (P < 0.05), although normal sperm morphology (MOR) did not differ (P > 0.05). In Experiment 2, after ejaculates extended with 20% EYP were cooled at 5°C for 2, 6 and 10 h before freezing, MOT, INT and ACR were similar among periods (P > 0.05). Thus, dog spermatozoon extended with 20% EYP can be kept cooled for up to 10 h prior to freezing, achieving post-thawing quality greater than that obtained with the inclusion of EY in freezing extenders.

References

Dec 1, 1974·Journal of Animal Science·M M Pace, E F Graham
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·D BencharifD Tainturier
Dec 23, 2008·Animal Reproduction Science·A S Varela JuniorJ C Deschamps

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Citations

Feb 25, 2020·Reproduction in Domestic Animals = Zuchthygiene·Djemil Bencharif, Marta Dordas-Perpinya
May 31, 2019·Reproduction in Domestic Animals = Zuchthygiene·Réda BelalaDjemil Bencharif
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Feriel Yasmine Mahiddine, Min-Jung Kim

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