Effect of eicosapentaenoic acid on glucose-induced diacylglycerol synthesis in cultured bovine aortic endothelial cells

Biochemical and Biophysical Research Communications
T KurokiH Nawata

Abstract

Hyperglycemia has been postulated to increase diacylglycerol (DAG) level through de novo synthesis pathway and subsequently activate protein kinase C (PKC) in vascular cells, possibly leading to vascular dysfunction associated with diabetes. In this study, we examined the effect of eicosapentaenoic acid (EPA) on high glucose-induced increase in DAG level in cultured aortic endothelial cells (ECs). In ECs, total DAG level was significantly increased in the cells cultured with high glucose levels (400 mg/dl) compared with the cells with normal glucose levels (100 mg/dl). The addition of EPA completely prevented high glucose-induced increase in total DAG level. In contrast, other common fatty acids such as palmitate and oleate significantly stimulated DAG syntheisis, although arachidonate did not affect it. High glucose level significantly stimulated the incorporation of 3H-palmitate into DAG, while it did not affect the incorporation of 3H-arachidonate into DAG. The addition of EPA completely prevented the high glucose-induced increase in 3H-palmitate incorporation into DAG, while it did not affect the 3H-arachidonate incorporation. These findings suggest that EPA can prevent high glucose induced-increase in DAG level in ECs, pro...Continue Reading

References

Jan 1, 1991·The Journal of Clinical Investigation·B A WolfJ Turk
Sep 1, 1989·Metabolism: Clinical and Experimental·T InoguchiH Nawata
May 1, 1989·The Journal of Clinical Investigation·P A Craven, F R DeRubertis

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Citations

Jun 1, 2010·Journal of Diabetes Investigation·Munehiro KitadaGeorge L King
Nov 24, 2004·Progress in Retinal and Eye Research·John Paul SanGiovanni, Emily Y Chew
Dec 1, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S MadaniN A Khan
Oct 30, 2003·The Journal of Biological Chemistry·Sihem MadaniNaim A Khan
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Toyoshi InoguchiHajime Nawata
Jan 26, 2007·Diabetes Care·Raffaele De CaterinaErik Berg Schmidt

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