PMID: 8591792Nov 1, 1995Paper

Effect of elastase on the directional migration of lung fibroblasts within a three-dimensional collagen matrix

Experimental Lung Research
A ChettyM Infeld

Abstract

Interactions between airway epithelial cells and bronchial fibroblasts often require close proximity between these cells. Previous studies have demonstrated that airway epithelial cells direct the migration of lung fibroblasts, but the factors that regulate this process during airway injury are not clear. We hypothesized that exposure of culture substrates to proteolytic enzymes, like those present in the inflamed airway, would increase fibroblast recruitment. We also postulated that elastase might affect the epithelium's ability to attract fibroblasts. We used an in vitro model with fibroblasts embedded between two layers of collagen gel to investigate their migration. Embedded fibroblasts exposed to culture medium alone (baseline) had a slight downward migration (migration directed to the upper gel layer expressed as a percentage of total migration was -2.8 +/- 1.4), but medium supplemented with porcine pancreatic elastase (PPE) resulted in a slight upward migration (2.0 +/- 1.4). When airway epithelial cells were cultured on the upper gel surface, the index of directed migration toward them was 15.9 +/- 1.3. Addition of PPE to the culture medium resulted in a significant increase to 22.3 +/- 1.5 (p < .05). Human neutrophil e...Continue Reading

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Citations

Mar 11, 2004·Life Sciences·Chien-Da HuangHan-Pin Kuo
Feb 26, 1998·American Journal of Respiratory and Critical Care Medicine·A M VignolaG Bonsignore
Aug 12, 1998·American Journal of Respiratory and Critical Care Medicine·I DurieuY Pacheco
Apr 8, 1999·American Journal of Respiratory and Critical Care Medicine·C M SköldS I Rennard
Nov 26, 1999·American Journal of Respiratory Cell and Molecular Biology·M J EvansC G Plopper
May 15, 2013·Molecular Immunology·Li ZuoKatherine S Salisbury
Dec 19, 2006·Biophysical Journal·Christopher B RaubSteven C George
Jun 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Megan E SummersSusan M Majka

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