PMID: 22574565May 12, 2012Paper

Effect of electroacupuncture on expression of gamma-glutamylcysteine synthetase protein and mRNA in cerebral cortex in rats with focal cerebral ischemia-reperfusion

Zhen ci yan jiu = Acupuncture research
Mei-Hong ShenZhong-Ren Li

Abstract

To observe the effect of electroacupuncture (EA) of "Baihui" (GV 20) and "Dazhui" (GV 14) on the expression of gamma-glutamylcysteine synthetase (gamma-GCS) protein and gene in the cerebral cortex in rats with cerebral ischemia-reperfusion (CI/R) injury, so as to explore its molecular biological mechanism underlying anti-oxidative stress. A total of 30 male Sprague-Dawley rats were randomized into sham operation (sham, n = 10), model (n = 10), and EA (n = 10) groups. CI/R model was established by right middle cerebral artery occlusion (modified Longa's thread occlusion method) for 2 hours and reperfusion for 24 hours. EA (3 Hz, 1-3 mA) was applied to "Dazhui" (GV 14) and "Baihui" (GV 20) for 30 min. gamma-GCS protein expression of the parietotemporal region of cerebral cortex was detected by immunohistochemistry and gamma-GCS heavy subunit (gamma-GCSh) mRNA and gamma-GCS light subunit (gamma-GCSI) mRNA expression levels were assayed by real-time quantitative polymerase chain reaction (RT-PCR). Compared with the sham group, the expression levels of gamma-GCS protein in the pyramidal cell layer of the cerebral cortical parietotemporal region, and y-GCSh mRNA and gamma-GCSI mRNA, and the number of gamma-GCS immuno-reaction positiv...Continue Reading

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.