Effect of electroacupuncture on silent information regulator 1, fork head transcription factor O1 and proopiomelanocortin in the hypothalamus of rats with obesity induced by high-fat diet

Zhen ci yan jiu = Acupuncture research
Yan Juan SongJia Feng Ren

Abstract

To investigate the effect of electroacupuncture (EA) on silent information regulator 1 (SIRT1), forkhead transcription factor O1 (FoxO1), and proopiomelanocortin (POMC) in the hypothalamus of rats with high-fat diet-induced obesity (DIO), as well as the mechanism of EA in regulating central appetite peptides to help lose weight. Male Wistar rats were randomly divided into normal group, model group, EA group, EA+inhibitor group, inhibitor group, and sham-operation group, with 10 rats in each group. High-fat diet was used to establish a rat model of DIO. The rats in the EA group and EA+inhibitor group were given EA at "Fenglong" (ST40), "Zhongwan "(CV12),"Guanyuan "(CV4), and"Zusanli" (ST36) with continuous wave at a frequency of 2 Hz and an intensity of 1 mA, for 10 minutes each time. The rats in the EA+inhibitor group and inhibitor group were given tube placement in the third ventricle and injection of the specific SIRT1 antagonist EX-527. The rats in the sham-operation group were given tube placement in the third ventricle and injection of artificial cerebrospinal fluid. The above treatment was given 3 times a week for 8 weeks in total. Body weight, food intake, and Lee's index were observed before and after treatment. An auto...Continue Reading

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