Effect of elite physical exercise by triathletes on seven catabolites of DNA oxidation

Free Radical Research
D VillañoA Gil-Izquierdo

Abstract

The oxidized nucleoside 8-hydroxy-2'-deoxyguanosine has been widely studied as a marker of DNA oxidation; however, data on the occurrence of other metabolites in plasma that are related to DNA damage are scarce. We have applied an improved, sensitive, robust, and reliable method, involving solid phase extraction and ultrahigh-performance liquid chromatography (UHPLC)-tandem mass spectrometry (MS/MS), to the precise quantitation of seven metabolites in the plasma of 15 elite triathletes after a 2-week training program. All compounds were eluted in the first 1.6 min, with limits of detection and quantification ranging between 0.001 and 0.3 ng.mL(-1) and 0.009 and 0.6 ng.mL(-1), respectively. Four compounds were detected in plasma: guanosine-3'-5'-cyclic monophosphate, 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanosine, and 8-nitroguanosine. After two weeks of training, 8-hydroxyguanine exhibited the highest increase (from 0.031 ± 0.008 nM to 0.036 ± 0.012 nM) (p < 0.05), which could be related to the enhanced activity of DNA-repairing enzymes that excise this oxidized base. Increased levels of guanosine-3'-5'-cyclic monophosphate and 8-hydroxy-2'-deoxyguanosine were also observed. In contrast, levels of 8-nitroguanosine (p < 0.05) wer...Continue Reading

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Citations

Apr 7, 2016·Food & Function·Libia Alejandra García-FloresÁngel Gil-Izquierdo
Mar 23, 2018·Acta Pharmacologica Sinica·Viktória VargaFerenc Domoki
Mar 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiung-Wen HuMu-Rong Chao
Mar 7, 2019·Cancer Investigation·Bilge Debelec-ButunerKemal Sami Korkmaz
Dec 7, 2020·Electrophoresis·Asmin AndriesAnn Van Schepdael
Dec 28, 2019·Journal of the American Chemical Society·Aaron M Fleming, Cynthia J Burrows

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