PMID: 16634282Apr 26, 2006Paper

Effect of eluent pH on the HPLC-UV analysis of hyperforin from St. John's Wort (Hypericum perforatum L.)

Phytochemical Analysis : PCA
Jean-Dominique Fourneron, Y Naït-Si

Abstract

The effect of the pH of the mobile phase in HPLC analysis of hyperforin was investigated. Working with an extract of St. John's Wort (Hypericum perforatum L.) that is rich in hyperforin, significant differences were observed in conventional chromatograms depending on whether the mobile phase was acidic or alkaline. Chromatogram changes were paralleled by changes in the UV spectrum of the hyperforin peak. The structural changes in hyperforin occur in the chromatographic column itself, as has been confirmed by UV spectroscopy performed on a sample of purified hyperforin, which showed that the UV spectrum is indeed dependent on the pH of its environment.

References

Jul 31, 1998·Pharmacopsychiatry·S S ChatterjeeC Erdelmeier
Apr 27, 1999·The Journal of Pharmacy and Pharmacology·H C OrthP C Schmidt
Jan 17, 2002·Phytochemical Analysis : PCA·A PoutaraudB Weniger
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Jun 6, 2003·Pharmazie in unserer Zeit·Veronika Butterweck, Adolf Nahrstedt
Feb 15, 2005·Journal of Pharmaceutical and Biomedical Analysis·Frances LiuCatharina Y W Ang

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Citations

Apr 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandar RaškovićMomir Mikov
Mar 8, 2017·The Journal of Pharmacy and Pharmacology·Anastasia AgapoudaDezső Csupor

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