Effect of embryo density on in vitro developmental characteristics of bovine preimplantative embryos with respect to micro and macroenvironments

Reproduction in Domestic Animals = Zuchthygiene
Michael HoelkerDawit Tesfaye

Abstract

To overcome developmental problems as a consequence of single embryo culture, the Well of the Well (WOW) culture system has been developed. In this study, we aimed to examine the effect of embryo densities with respect to both microenvironment and macroenvironment on developmental rates and embryo quality to get a deeper insight into developmentally important mechanisms. WOW diameter and depth significantly affected developmental rates (p < 0.05). WOWs with diameter of 500 μm reached significantly higher blastocyst rates (32.5 vs 21.1% vs 20.3%) compared to embryos cultured in WOWs of 300 μm diameter or plain cultured controls. Embryos cultured in WOWs with 700 μm depth reached significant higher developmental rates compared with embryos cultured in WOWs of 300 μm depth and control embryos (30.6 vs 22.6% vs 20.3%). Correlation of the embryo per WOW volume with developmental rates was higher (r(2) = 0.92, p = 0.0004) than correlation of WOW diameter or WOW depth with developmental rates. However, the embryo per WOW volume did not affect differential cell counts. An embryo per culture dish volume of 1 : 30 μl was identified to be optimal when the embryo per WOW volume was 1 : 0.27 μl increasing developmental rates up to the level...Continue Reading

References

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Citations

Dec 15, 2012·Journal of Assisted Reproduction and Genetics·Tao TaoRodney Ouellette
Apr 1, 2014·Journal of Assisted Reproduction and Genetics·V A Absalón-MedinaR O Gilbert
Jun 7, 2011·Reproductive Biomedicine Online·Vladimir IsachenkoEvgenia Isachenko
Jul 7, 2018·International Journal of Biomaterials·Daisaku IwamotoKazuhiro Saeki
Oct 17, 2017·The Journal of Reproduction and Development·Yutaka Hashiyada
Nov 17, 2020·Frontiers in Veterinary Science·Ahmad Yar QamarJongki Cho

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