Effect of endothelin-1 on lipolysis in rat adipocytes

Obesity
C C JuanLow-Tone Ho

Abstract

To explore the role of endothelin-1 (ET-1) on lipid metabolism, we examined the effect of ET-1 on lipolysis in rat adipocytes. Adipocytes isolated from male Sprague-Dawley rats, weighing 400 to 450 grams, were incubated in Krebs-Ringer buffer with or without 10(-7) M ET-1 for various times or with various concentrations of ET-1 for 4 hours; then glycerol release into the incubation medium was measured. In addition, selective ET(A)R and ET(B)R blockers were used to identify the ET receptor subtype involved. We also explored the involvement of cyclic adenosine monophosphate (cAMP) in ET-1-stimulated lipolysis using an adenylyl cyclase inhibitor and by measuring changes in intracellular cAMP levels in response to ET-1 treatment. To further explore the underlying mechanism of ET-1 action, we examined the involvement of the extracellular signal-regulated kinase (ERK)-mediated pathways. Our results showed that ET-1 caused lipolysis in rat adipocytes in a time- and dose-dependent manner. BQ610, a selective ET(A)R blocker, blocked this effect. The adenylyl cyclase inhibitor, 2',5'-dideoxyadenosine, had no effect on ET-1-stimulated lipolysis. ET-1 did not induce an increase in intracellular cAMP levels. In addition, ET-1-induced lipolys...Continue Reading

References

Sep 1, 1992·Zentralblatt für Veterinärmedizin. Reihe A·R Rossi, E Scharrer
Mar 1, 1992·Metabolism: Clinical and Experimental·M RodenW Waldhäusl
Aug 5, 1991·FEBS Letters·K HosodaH Imura
Jun 14, 1991·Biochemical and Biophysical Research Communications·T TanahashiO Abe
Jul 29, 1994·Biochemical and Biophysical Research Communications·Y C ChouL T Ho
Mar 8, 1993·FEBS Letters·T A Tran-ThiK Decker
Jun 15, 1996·The Journal of Clinical Investigation·M RodenG I Shulman
Jan 1, 1997·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·C FerriG De Mattia
Apr 4, 2000·American Journal of Respiratory Cell and Molecular Biology·S I AhmedP J Woll
Aug 15, 2000·Annual Review of Nutrition·C HolmJ A Contreras
May 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J L LiuI H Zucker
May 22, 2001·The Journal of Biological Chemistry·Y XiongM Yanagisawa
Aug 7, 2002·Biochemical and Biophysical Research Communications·Qiao ZhongRobert L Judd
Nov 21, 2002·American Journal of Hypertension·Jochen G SchneiderChristian Kasperk
Jan 18, 2003·The Journal of Clinical Investigation·Katrin HückingRichard N Bergman
Jul 29, 2003·Diabetes·Jason J WilkesJerrold Olefsky
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·L C C NavegantesP Lönnroth
Dec 4, 2003·Biochemical and Biophysical Research Communications·Kristen J ClarkeRobert L Judd
Dec 11, 2003·American Journal of Physiology. Endocrinology and Metabolism·Seiji MaedaMitsuo Matsuda
Mar 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cedric MoroMichel Berlan
Jun 8, 2004·The Journal of Clinical Endocrinology and Metabolism·Erin E Kershaw, Jeffrey S Flier
Oct 7, 2004·Neuropeptides·María del Rosario Garrido, Anita Israel
Jan 27, 2005·American Journal of Physiology. Endocrinology and Metabolism·Chi-Chang JuanLow-Tone Ho

❮ Previous
Next ❯

Citations

May 8, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·D Yesica GarciafigueroaAaron Barchowsky
May 30, 2014·Nutrition Research Reviews·Gema FrühbeckAmaia Rodríguez
Apr 25, 2009·BioFactors·Kepeng Wang, Yung H Wong
Jun 11, 2011·Biochimie·Valéria Ernestânia ChavesNair Honda Kawashita
Mar 24, 2015·PloS One·Vanesa Martínez-BarqueroFelipe Javier Chaves
Jun 24, 2014·Biochemical Pharmacology·Jonathon C B DaviesVenkateswarlu Kanamarlapudi
Nov 5, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A K S ErikssonM Rydén
Jul 5, 2011·Journal of the Science of Food and Agriculture·Ron PoratArnon Dag
Jul 17, 2019·American Journal of Physiology. Endocrinology and Metabolism·Thomas J JurrissenJaume Padilla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved