PMID: 7030066Sep 1, 1981Paper

Effect of endotoxin on arachidonic acid release and thromboxane B2 production by human platelets

American Journal of Hematology
M J Stuart

Abstract

Plasma thromboxane A2, which is elevated during endotoxemia, has previously been shown to be a major factor contributing to the mortality and morbidity that occurs in endotoxin shock in the experimental animal. Using a minimal dose of Escherichia coli endotoxin (1 microgram/ml), we have demonstrated that the preincubation of human platelets with endotoxin induces changes in platelet arachidonic acid release and the subsequent conversion of the released arachidonic acid to thromboxane B2, and stable end product of thromboxane A2. In paired experiments, in the presence of endotoxin, the addition of the aggregating agent thrombin (0.5 U/ml) caused human platelets to release 29.1 +/- 3.4% of 14C-arachidonic acid from prelabeled platelet phospholipids. This value was significantly elevated (p less than 0.02) when compared with the release of 14C-arachidonic acid from platelets in the absence of endotoxin (21.9 +/- 3.6%). Similarly, comparison of the results of the conversion of the released arachidonic acid to platelet thromboxane B2 (TxB2) revealed that TxB2 production was significantly increased (P less than 0.01) when human platelets were preincubated with endotoxin prior to the addition of thrombin (6.1 +/- 0.6%) when compared w...Continue Reading

References

Jul 1, 1977·Prostaglandins·J M GerrardJ G White
Jul 10, 1975·Nature·J HawigerS Timmons
Feb 23, 1976·Biochimica Et Biophysica Acta·T K BillsM J Silver
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson

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Citations

Sep 1, 1985·Inflammation·J T RosenbaumI M Goldstein
Jan 1, 1986·Bio Systems·S D VarfolomeevA T Mevkh
Aug 1, 1990·Journal of Clinical Periodontology·J Syrjänen
Mar 25, 1989·BMJ : British Medical Journal·K J MattilaM J Jokinen
Sep 1, 1988·Infection·R Lorenz, M Brauer
Jan 1, 1984·The American Journal of Emergency Medicine·E T RietschelU Schade
Mar 1, 1989·Journal of Internal Medicine·J SyrjänenJ K Huttunen

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