Effect of EPA on Neonatal Pig Sertoli Cells "In Vitro": A Possible Treatment to Help Maintain Fertility in Pre-Pubertal Boys Undergoing Treatment With Gonado-Toxic Therapies.

Frontiers in Endocrinology
Iva AratoGiovanni Luca

Abstract

The incidence of cancer in pre-pubertal boys has significantly increased and, it has been recognized that the gonado-toxic effect of the cancer treatments may lead to infertility. Here, we have evaluated the effects on porcine neonatal Sertoli cells (SCs) of three commonly used chemotherapy drugs; cisplatin, 4-Hydroperoxycyclophosphamide and doxorubicin. All three drugs induced a statistical reduction of 5-hydroxymethylcytosine in comparison with the control group, performed by Immunofluorescence Analysis. The gene and protein expression levels of GDNF, were significantly down-regulated after treatment to all three chemotherapy drugs comparison with the control group. Specifically, differences in the mRNA levels of GDNF were: 0,8200 ± 0,0440, 0,6400 ± 0,0140, 0,4400 ± 0,0130 fold change at 0.33, 1.66, and 3.33μM cisplatin concentrations, respectively (**p < 0.01 at 0.33 and 1.66 μM vs SCs and ***p < 0.001 at 3.33μM vs SCs); 0,6000 ± 0,0340, 0,4200 ± 0,0130 fold change at 50 and 100 μM of 4-Hydroperoxycyclophosphamide concentrations, respectively (**p < 0.01 at both these concentrations vs SCs); 0,7000 ± 0,0340, 0,6200 ± 0,0240, 0,4000 ± 0,0230 fold change at 0.1, 0.2 and 1 µM doxorubicin concentrations, respectively (**p < 0.01...Continue Reading

References

Jun 1, 1984·The Journal of Urology·T W HensleJ H Wigger
Jul 15, 1998·Human Reproduction·D M de KretserN Wreford
Nov 18, 2000·Journal of Endocrinological Investigation·P RossiR Geremia
Nov 27, 2001·European Journal of Endocrinology·S J MeachemM Simoni
May 4, 2002·Molecular and Cellular Endocrinology·Stefan Schlatt
Jul 12, 2003·Biology of Reproduction·Luis DettinMartin Dym
Sep 24, 2004·Psycho-oncology·N E LangeveldC van den Bos
Feb 15, 2005·Developmental Biology·Marie-Claude HofmannMartin Dym
Mar 24, 2005·Journal of the National Cancer Institute. Monographs·Leslie R Schover
Oct 21, 2005·Biology of Reproduction·Cathy K NaughtonJeffrey Milbrandt
Feb 1, 2006·Human Reproduction Update·Jens EhmckeStefan Schlatt
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·C C OakesJ M Trasler
Dec 20, 2007·Growth Factors·Mahmoud HuleihelEitan Lunenfeld
May 20, 2008·Molecular and Cellular Endocrinology·Marie-Claude Hofmann
Mar 26, 2009·The Journal of Clinical Endocrinology and Metabolism·Mirja NurmioKirsi Jahnukainen
Oct 1, 2009·The Journal of Clinical Endocrinology and Metabolism·Patrik RomeriusAleksander Giwercman
Dec 2, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel M GreenLeslie L Robison
Jan 6, 2010·Human Reproduction Update·Christine WynsJacques Donnez
Mar 6, 2010·International Journal of Urology : Official Journal of the Japanese Urological Association·Gert R Dohle
Mar 17, 2010·Cancer Treatment Reviews·Peter Kaatsch
Jun 3, 2010·Cold Spring Harbor Protocols·Donald C RioTimothy W Nilsen
Mar 15, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·Kirsi JahnukainenStefan Schlatt
Mar 18, 2011·Basic & Clinical Pharmacology & Toxicology·Ana Paula Alves FavaretoWilma De Grava Kempinas
Nov 16, 2011·Nature Reviews. Genetics·Miguel R BrancoWolf Reik
Feb 1, 2012·Fertility and Sterility·Douglas T Carrell
Apr 1, 2011·Journal of Adolescent and Young Adult Oncology·Caprice A KnappDevin Murphy
Nov 5, 2013·Fertility and Sterility·Edward DereKim Boekelheide
Dec 21, 2013·Hormone Research in Pædiatrics·Jan-Bernd StukenborgOlle Söder
Mar 8, 2014·Seminars in Cell & Developmental Biology·Gurvinder KaurJannette M Dufour
Apr 9, 2014·Journal of Cancer Survivorship : Research and Practice·K Wasilewski-MaskerL R Meacham
Dec 17, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Su-Ren Chen, Yi-Xun Liu
Jan 7, 2016·Hormones : International Journal of Endocrinology and Metabolism·Fotios DimitriadisNikolaos Sofikitis
Jan 10, 2016·Fertility and Sterility·Kathrin Gassei, Kyle E Orwig
May 27, 2016·CNS Neuroscience & Therapeutics·Giovanni LucaFerdinando Squitieri
Jun 3, 2016·CA: a Cancer Journal for Clinicians·Kimberly D MillerAhmedin Jemal
Feb 6, 2017·BMJ : British Medical Journal·Rod T MitchellGwendolyn P Quinn

❮ Previous
Next ❯

Software Mentioned

VersaDoc Imaging System
Cell F
Systat
Quantity One
SigmaStat

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Pain Practice : the Official Journal of World Institute of Pain
Ricardo VallejoRamsin Benyamin
© 2021 Meta ULC. All rights reserved