Effect of epiberberine from Coptis chinensis Franch on inhibition of tumor growth in MKN-45 xenograft mice

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Min YuXiaoli Ye

Abstract

Gastric cancer is one of the major malignancies worldwide. Epiberberine (EPI) is a major alkaloid from Coptis chinensis Franch and the antitumor property of EPI remains poorly understood. The inhibition on gastric cancer cells was observed by MTT assays and colony formation experiments. The apoptosis, cell cycle, and reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) in gastric cancer cells were analyzed by Flow cytometry. The anti-tumor effect of EPI was evaluated with the MKN-45-beraring nude mice, and the potential mechanisms were explored by RNA-seq, qPCR, siRNA silencing and western blotting. EPI inhibited the proliferation of human gastric cancer cell lines MKN-45 (harboring wild-type p53) and HGC-27 (harboring mutant p53) in a dose dependent manner. EPI induced the apoptosis and cell cycle arrest in these two cell lines, of which MKN-45 cells are more sensitive to EPI than HGC-27 cells. Further experiments indicated that EPI induced the accumulation of ROS and decreased of ΔΨm in MKN-45 cells. The significant differentially expressed genes obtained by RNA-seq were distinctly enriched in the p53 signaling pathway. The apoptosis induced by EPI in MKN-45 cells would be effectively inhibited with the tr...Continue Reading

Citations

Oct 8, 2020·Chinese Medicine·Chunhao ZhuJianqiang Yu
Nov 11, 2020·International Journal of Molecular Sciences·Sylwia NakoniecznaWirginia Kukula-Koch
Feb 2, 2021·Journal of Ethnopharmacology·Yuling TongYanling Zhao
Feb 14, 2021·European Journal of Medicinal Chemistry·Dong-Jun FuBin Yu
Nov 6, 2020·Fitoterapia·Linqiu LiuYang He

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