Effect of epidermal growth factor in collagen gel cultures of rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
K de SmetVera Rogiers

Abstract

Collagen gel cultures of hepatocytes represent a promising in vitro model in pharmaco-toxicology. Epidermal growth factor (EGF) is usually added to the culture medium, although one could question its value in a culture model aiming at maintaining a maximum of differentiated functional capacities. In this study, the effects of EGF (20 ng/ml) on albumin secretion, morphology and pentoxyresorufin O-depentylase (PROD), ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) activities have been examined in both collagen gel sandwich and immobilization gel cultures of adult rat hepatocytes. Transmission electron microscopy did not show an obvious influence of epidermal growth factor (EGF) on the intracellular organization of organelles of the rat hepatocytes. It was found that EGF addition had no effect on albumin secretion in both culture models. On the contrary, the presence of EGF in the culture medium provoked in collagen gel sandwich cultures, after 7 days, significant decreases of 66% and 25% in EROD and PROD activities, respectively. On GST activities, no effect of EGF could be observed in both collagen gel cultures. Removal of EGF from the culture medium seemed to have a positive effect on the maintenance of ...Continue Reading

References

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Oct 28, 2003·Methods in Molecular Biology·K De SmetV Rogiers
Apr 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RotemM L Yarmush
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BekenV Rogiers
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K de SmetV Rogiers
Dec 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BekenV Rogiers

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Citations

Feb 2, 2012·Journal of Nutritional Science and Vitaminology·Noriko YamaguchiKiwamu Yoshikawa
May 29, 2012·Expert Opinion on Drug Metabolism & Toxicology·Ajit DashBrian R Wamhoff
May 24, 2016·Journal of Pharmaceutical Sciences·Christian Kölln, Stephan Reichl
Jan 24, 2014·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Carrie A Schroeder, Rebecca A Johnson
Mar 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dora Farkas, Steven R Tannenbaum
Mar 9, 2006·Alternatives to Laboratory Animals : ATLA·Sandra CoeckeAndrew Worth
Mar 15, 2013·American Journal of Physiology. Cell Physiology·A DashB R Wamhoff

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