Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos.

Biology of Reproduction
Angelica M GiraldoKenneth R Bondioli

Abstract

Evidence indicates that failure of nuclear transfer (NT) embryos to develop normally can be attributed, at least partially, to the use of differentiated cells as the donor karyoplast. Blastocyst production and development to term of cloned embryos has been hypothesized to differ between population doublings of the same cell line as a consequence of changes in the levels of DNA methyltransferase 1 (DNMT1) and methylated DNA during in vitro culture. The objective of this study was to determine embryo production, developmental potential, and gene expression patterns of prehatched and posthatched embryos generated using donor cells with different levels of DNMT1 transcript. Day 7 embryos generated using donor cells with high and low levels of DNMT1 mRNA were transferred to recipient cows. Embryos recovered on Day 13 were morphologically characterized or used for gene expression analysis of DNMT, INFT, and MHC1. A higher proportion of 8- to 16-cell embryos developed to the blastocyst stage when cells with low levels of DNMT1 mRNA were used as donor nuclei. Day 13 NT embryos generated using donor cells with decreased levels of DNMT1 mRNA and capable of developing beyond the 8- to 16-cell stage produced a larger number of apparently d...Continue Reading

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Citations

Jul 2, 2009·Reproduction, Fertility, and Development·Angelica M GiraldoKenneth R Bondioli
Mar 8, 2012·Reproduction, Fertility, and Development·Olivier SandraRichard G Lea
Jan 19, 2011·Cellular Reprogramming·Joana Claudia MezzaliraMarcelo Bertolini
Sep 12, 2013·Cellular Reprogramming·Jaroslaw StaszkiewiczKenneth J Eilersten
Oct 10, 2009·The Journal of Reproduction and Development·Ken-Ichi YamanakaMasashi Takahashi
Jun 2, 2010·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jie LanYong Zhang
Sep 18, 2012·Theriogenology·N Rodriguez-OsorioE Memili
Apr 23, 2013·Biochemical and Biophysical Research Communications·Yongzhi TianMingjun Liu
Jul 3, 2013·Cellular Reprogramming·Angelica M GiraldoDavid L Ayares
Oct 19, 2010·Biologie aujourd'hui·Nathalie BeaujeanPascale Debey
Dec 15, 2019·Reproduction in Domestic Animals = Zuchthygiene·Yumei ZhangJian Hou
May 26, 2009·Ageing Research Reviews·Alexander L Mazin

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