PMID: 20134117Feb 6, 2010Paper

Effect of erythropoietin on angiogenesis with the increased adhesion of platelets to the microvessels in the hind-limb ischemia model in mice

Journal of Pharmacological Sciences
Shintaro KatoMasataka Majima

Abstract

Erythropoietin (EPO) has been shown to enhance angiogenesis, but its precise mechanisms of enhancement during ischemia are not fully elucidated. We examined the effect of EPO on blood flow recovery from acute hind-limb ischemia induced by ligation of the femoral artery in male C57Bl/6 mice. The density of microvessels with platelet adhesion in ischemic tissues was assessed by intravital microscopy. Treatment with EPO (100 and 1000 IU/kg, i.p.) restored blood flow in a dose-dependent manner and increased plasma levels of soluble-P-selectin, vascular endothelial growth factor (VEGF), and stromal cell-derived factor (SDF-1). Flow cytometric analysis revealed increased P-selectin expression on platelets in EPO-treated mice compared to PBS-treated mice. Intravital microscopic studies showed that EPO increased density of microvessels with platelet adhesion selectively in the ischemic tissues. Neutralizing antibody against P-selectin reduced the density of microvessels with platelet adhesion enhanced with EPO and impaired blood flow recovery with reductions in VEGF and SDF-1 levels. These results suggest that EPO administration enhances recovery from hind-limb ischemia, and platelet adhesion to the microvessels is a key event to enhan...Continue Reading

References

Dec 16, 1998·Lancet·H M PinedoJ Folkman
Dec 24, 1998·British Journal of Pharmacology·E Pipili-SynetosM E Maragoudakis
Jul 30, 2005·Circulation Research·Hideki AmanoRonald G Crystal
Dec 3, 2005·The Journal of Clinical Investigation·Meinrad GawazAndreas E May
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Janka KisuckaDenisa D Wagner
Apr 28, 2006·Journal of Leukocyte Biology·Kimiyasu EgamiToyojiro Matsuishi
Mar 14, 2007·British Journal of Pharmacology·T E Peterson, Z S Katusic
Mar 21, 2007·Hypertension·Livius V d'UscioZvonimir S Katusic
Mar 31, 2007·Annals of the New York Academy of Sciences·H G Kopp, S Rafii
Jun 15, 2007·Trends in Immunology·Isabelle PetitShahin Rafii
Apr 9, 2008·Basic Research in Cardiology·Harald F Langer, Meinrad Gawaz

❮ Previous
Next ❯

Citations

Mar 10, 2012·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Kivanc AtesokEmil Schemitsch
Jun 12, 2014·International Journal of Molecular Sciences·Yuanyuan ZhangConstance Tom Noguchi
Dec 21, 2011·The Journal of Physiology·Karen Y Stokes, D Neil Granger
Nov 16, 2010·The Journal of Surgical Research·Christian EipelBrigitte Vollmar
Feb 19, 2011·Thrombosis and Haemostasis·L Zagorchev, M J Mulligan-Kehoe
May 4, 2015·Cardiovascular Research·Hideki AmanoMasataka Majima
Dec 21, 2010·Biochimica Et Biophysica Acta·Siamack SabrkhanyMirjam G A Oude Egbrink
Dec 19, 2012·The Canadian Journal of Cardiology·Ozgür Günebakmaz, Mehmet G Kaya
Apr 23, 2017·Seminars in Immunology·Ashwin Nair, Liping Tang
Jul 14, 2017·International Journal of Molecular Sciences·Patrícia KimákováNataša Debeljak
Nov 14, 2019·British Journal of Pharmacology·Maximilian M MengerEmmanuel Ampofo
Dec 7, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Toshiaki MishimaMasataka Majima
Mar 10, 2012·Reproduction, Fertility, and Development·L ComminV Neto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.