PMID: 6340723Feb 1, 1983

Effect of Escherichia coli initiation factors on the kinetics of N-Acphe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study

Biochemistry
Wolfgang Wintermeyer, C O Gualerzi

Abstract

The mechanism of binding of N-AcPhe-tRNAPhe (yeast) to poly(U)-programmed Escherichia coli 30S ribosomal subunits and the effect of individual initiation factors (IF-1, IF-2, and IF-3) and GTP on this process have been studied by fluorescence stopped-flow kinetic measurements. The formation of the ternary complex was followed by an increase of both intensity and polarization of the fluorescence of a proflavin label located in the anticodon loop of the tRNA. The effect of the initiation factors and GTP is to increase the velocity of ternary complex formation (about 400-fold at 7 mM Mg2+). In the presence of the three initiation factors and GTP the formation of the ternary complex could be resolved into two partial reactions: a fast apparently second-order step (k12 = 5 x 10(6) M-1 s-1, k21 = 1.4 s-1) followed by a slow rearrangement step (k23 less than or equal to 0.1 s-1). The data suggest a mechanism in which the ternary complex is formed by at least two rearrangements of an initially formed preternary complex. The accelerating effects of both IF-2 and IF-3 can be understood by assuming a synergistic allosteric action of the factors on the 30S ribosomal subunit, whereas IF-1 appears to act indirectly by influencing the other t...Continue Reading

References

Aug 1, 1979·European Journal of Biochemistry·Wolfgang Wintermeyer, H G Zachau
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B M BlumbergF J Kézdy
Jul 1, 1979·European Journal of Biochemistry·M LéonR H Buckingham
Dec 14, 1979·Biochemical and Biophysical Research Communications·H U PetersenB F Clark
Oct 8, 1974·Biochemical and Biophysical Research Communications·S D BernalT Nakamoto
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, F Lipmann
Sep 5, 1981·Journal of Molecular Biology·J M Robertson, Wolfgang Wintermeyer
Aug 5, 1980·Biochemistry·D Labuda, D Pörschke

Citations

Nov 11, 1999·Trends in Biotechnology·G A Soukup, R R Breaker
Jan 20, 2004·European Journal of Biochemistry·Victor CroitoruLeif A Isaksson
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aymen S YassinRajendra K Agrawal
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R A CalogeroClaudio Gualerzi
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·Marina V Rodnina, Wolfgang Wintermeyer
May 3, 2000·The EMBO Journal·J TomsicClaudio Gualerzi
Aug 14, 2001·The EMBO Journal·D PetrelliClaudio Gualerzi
Dec 15, 1988·European Journal of Biochemistry·B CelanoC O Gualerzi
Mar 27, 2007·Journal of Bacteriology·Sangita PhadtareKonstantin Severinov
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Apr 27, 2012·PLoS Genetics·K Elizabeth MadisonHiroshi Nakai
Oct 26, 2010·Journal of Biotechnology·Wenjie ShuShengqi Wang
Feb 27, 2009·Current Opinion in Cell Biology·Marina V Rodnina, Wolfgang Wintermeyer
Oct 28, 2008·Gene·Stavroula KapralouRoberto Spurio
Jun 6, 2003·Molecular Microbiology·Paula Jean Schlax, David J Worhunsky
Oct 21, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sangita Phadtare, Konstantin Severinov
Jan 24, 2007·Molecular Microbiology·Gregory S Allen, Joachim Frank
Feb 6, 2010·Molecular Microbiology·Anna ZorzetDan I Andersson
Oct 16, 2007·Biochemical and Biophysical Research Communications·Louise Carøe Vohlander RasmussenKim Kusk Mortensen
Aug 12, 2003·Journal of Molecular Biology·Maarten H de Smit, Jan van Duin
Mar 16, 2007·Structure·Tatsuya KaminishiShigeyuki Yokoyama
Jul 15, 2009·Molecular Cell·R Andrew MarshallJoseph D Puglisi
Mar 5, 1991·Journal of Molecular Biology·D HartzL Gold
Oct 4, 2017·Nucleic Acids Research·Yuanwei ChenBarry S Cooperman
May 26, 2006·The EMBO Journal·Ayman AntounMåns Ehrenberg
Apr 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Pohl Milón, Marina V Rodnina
Jun 3, 2017·Nucleic Acids Research·He ChengguangClaudio Gualerzi
May 29, 2018·Biotechnology & Genetic Engineering Reviews·Luc Roberts, Hans-Joachim Wieden
Jan 27, 2019·International Journal of Molecular Sciences·Anna Maria GiuliodoriClaudio Gualerzi

Related Concepts

TRNA, N-acetylphenylalanine-
Alkalescens-Dispar Group
Guanosine Triphosphate
Magnesium
Peptide Initiation Factors
Ribosomes
RNA, Transfer, Amino Acyl
Fluorescence Spectroscopy
Prokaryotic Initiation Factor-3

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.