PMID: 6340723Feb 1, 1983

Effect of Escherichia coli initiation factors on the kinetics of N-Acphe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study

Wolfgang Wintermeyer, C O Gualerzi


The mechanism of binding of N-AcPhe-tRNAPhe (yeast) to poly(U)-programmed Escherichia coli 30S ribosomal subunits and the effect of individual initiation factors (IF-1, IF-2, and IF-3) and GTP on this process have been studied by fluorescence stopped-flow kinetic measurements. The formation of the ternary complex was followed by an increase of both intensity and polarization of the fluorescence of a proflavin label located in the anticodon loop of the tRNA. The effect of the initiation factors and GTP is to increase the velocity of ternary complex formation (about 400-fold at 7 mM Mg2+). In the presence of the three initiation factors and GTP the formation of the ternary complex could be resolved into two partial reactions: a fast apparently second-order step (k12 = 5 x 10(6) M-1 s-1, k21 = 1.4 s-1) followed by a slow rearrangement step (k23 less than or equal to 0.1 s-1). The data suggest a mechanism in which the ternary complex is formed by at least two rearrangements of an initially formed preternary complex. The accelerating effects of both IF-2 and IF-3 can be understood by assuming a synergistic allosteric action of the factors on the 30S ribosomal subunit, whereas IF-1 appears to act indirectly by influencing the other t...Continue Reading


Aug 1, 1979·European Journal of Biochemistry·Wolfgang Wintermeyer, H G Zachau
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B M BlumbergF J Kézdy
Jul 1, 1979·European Journal of Biochemistry·M LéonR H Buckingham
Dec 14, 1979·Biochemical and Biophysical Research Communications·H U PetersenB F Clark
Oct 8, 1974·Biochemical and Biophysical Research Communications·S D BernalT Nakamoto
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, F Lipmann
Sep 5, 1981·Journal of Molecular Biology·J M Robertson, Wolfgang Wintermeyer
Aug 5, 1980·Biochemistry·D Labuda, D Pörschke


Nov 11, 1999·Trends in Biotechnology·G A Soukup, R R Breaker
Jan 20, 2004·European Journal of Biochemistry·Victor CroitoruLeif A Isaksson
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aymen S YassinRajendra K Agrawal
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R A CalogeroClaudio Gualerzi
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·Marina V Rodnina, Wolfgang Wintermeyer
May 3, 2000·The EMBO Journal·J TomsicClaudio Gualerzi
Aug 14, 2001·The EMBO Journal·D PetrelliClaudio Gualerzi
Dec 15, 1988·European Journal of Biochemistry·B CelanoC O Gualerzi
Mar 27, 2007·Journal of Bacteriology·Sangita PhadtareKonstantin Severinov
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Apr 27, 2012·PLoS Genetics·K Elizabeth MadisonHiroshi Nakai
Oct 26, 2010·Journal of Biotechnology·Wenjie ShuShengqi Wang
Feb 27, 2009·Current Opinion in Cell Biology·Marina V Rodnina, Wolfgang Wintermeyer
Oct 28, 2008·Gene·Stavroula KapralouRoberto Spurio
Jun 6, 2003·Molecular Microbiology·Paula Jean Schlax, David J Worhunsky
Oct 21, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sangita Phadtare, Konstantin Severinov
Jan 24, 2007·Molecular Microbiology·Gregory S Allen, Joachim Frank
Feb 6, 2010·Molecular Microbiology·Anna ZorzetDan I Andersson
Oct 16, 2007·Biochemical and Biophysical Research Communications·Louise Carøe Vohlander RasmussenKim Kusk Mortensen
Aug 12, 2003·Journal of Molecular Biology·Maarten H de Smit, Jan van Duin
Mar 16, 2007·Structure·Tatsuya KaminishiShigeyuki Yokoyama
Jul 15, 2009·Molecular Cell·R Andrew MarshallJoseph D Puglisi
Mar 5, 1991·Journal of Molecular Biology·D HartzL Gold
Oct 4, 2017·Nucleic Acids Research·Yuanwei ChenBarry S Cooperman
May 26, 2006·The EMBO Journal·Ayman AntounMåns Ehrenberg
Apr 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Pohl Milón, Marina V Rodnina
Jun 3, 2017·Nucleic Acids Research·He ChengguangClaudio Gualerzi
May 29, 2018·Biotechnology & Genetic Engineering Reviews·Luc Roberts, Hans-Joachim Wieden
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