PMID: 321457Apr 10, 1977Paper

Effect of estrogen on gene expression in the chick oviduct.

The Journal of Biological Chemistry
H C TowleB W O'Malley

Abstract

Mercurated UTP was used as a substrate for RNA polymerases in the in vitro transcription of chromatin so that newly synthesized RNA could be efficiently separated from endogenous chromatin RNA by means of sulfhydryl-Sepharose affinity chromatography. Utilizing this technique, it was possible to examine the effect of varying enzyme to DNA ratios on the transcription of specific genes from chromatin. For both Escherichia coli RNA polymerase and wheat germ RNA polymerase II, lowering the enzyme to DNA ration resulted in an increase in the percentage of ovalbumin mRNA sequences transcribed from chick oviduct chromatin. Similar results were also obtained for the transcription of the globin gene from chick reticulocyte chromatin. On the other hand, transcription of the globin gene from oviduct chromatin or the ovalbumin gene from reticulocyte chromatin or deproteinized chick DNA was not significantly affected by varying enzyme to DNA ratios. These results indicate that preferential transcription of certain chromatin genes relative to total RNA synthesis can occur and that this process is dependent on the presence of chromosomal proteins. Utilizing a cDNA probe complementary to the anticoding strand of the ovalbumin gene, the degree o...Continue Reading

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Citations

Apr 30, 1981·Developmental Biology·R B GoldbergR W Breidenbach
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M C Nguyen-HuuG Schütz
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R GangulyM R Banerjee
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G E SwaneckB W O'Malley
Oct 1, 1980·The Journal of Cell Biology·B A Brennessel, D K Biswas
Apr 1, 1978·Nucleic Acids Research·D A Konkel, V M Ingram
Sep 11, 1980·Nucleic Acids Research·H G HodoG F Saunders
Aug 1, 1980·Biological Reviews of the Cambridge Philosophical Society·J R Tata
Sep 1, 1979·FEBS Letters·D MaryankaH J Gould
Sep 1, 1980·European Journal of Biochemistry·B Teyssot, L M Houdebine
May 22, 1981·Molecular Biology Reports·M Hanausek-WalaszekM Chorazy

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