Effect of estrogens on boar sperm capacitation in vitro.

Reproductive Biology and Endocrinology : RB&E
Lukas DedJana Peknicova

Abstract

Mammalian sperm must undergo a series of controlled molecular processes in the female reproductive tract called capacitation before they are capable of penetrating and fertilizing the egg. Capacitation, as a complex biological process, is influenced by many molecular factors, among which steroidal hormone estrogens play their role. Estrogens, present in a high concentration in the female reproductive tract are generally considered as primarily female hormones. However, there is increasing evidence of their important impact on male reproductive parameters. The purpose of this study is to investigate the effect of three natural estrogens such as estrone (E1), 17beta-estradiol (E2) and estriol (E3) as well as the synthetical one, 17 alpha-ethynylestradiol (EE2) on boar sperm capacitation in vitro. Boar sperm were capacitated in vitro in presence of estrogens. Capacitation progress in control and experimental samples was analyzed by flow cytometry with the anti-acrosin monoclonal antibody (ACR.2) at selected times of incubation. Sperm samples were analyzed at 120 min of capacitation by CTC (chlortetracycline) assay, immunocytochemistry and flow cytometry with anti-acrosin ACR.2 antibody. Furthermore, sperm samples and capacitating ...Continue Reading

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Citations

Aug 27, 2013·General and Comparative Endocrinology·Giovanna CacciolaGilda Cobellis
Aug 30, 2011·Reproduction, Fertility, and Development·Marta PuigmuléElisabeth Pinart
Jan 16, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Lukas DedKaterina Dvorakova-Hortova
Apr 5, 2014·General and Comparative Endocrinology·Malgorzata Kotula-BalakBarbara Bilinska
Sep 13, 2016·Journal of Ginseng Research·Sandra L GrayWilliam R Boone
Apr 2, 2015·Reproductive Toxicology·Sandra L GrayWilliam R Boone
Aug 11, 2015·Expert Reviews in Molecular Medicine·Sara CorreiaSílvia Socorro
May 26, 2016·General and Comparative Endocrinology·Camille GautierHélène Bouraïma-Lelong
Aug 28, 2016·Reproductive Biology and Endocrinology : RB&E·Malgorzata KotwickaPiotr Jedrzejczak
Apr 26, 2017·International Journal of Molecular Sciences·Pavla DostalovaKaterina Dvorakova-Hortova
Dec 23, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Yuanxian WangWuzi Dong
Mar 9, 2011·Cell and Tissue Research·Pavla Maňásková-PostlerováVěra Jonáková
Dec 19, 2019·International Journal of Molecular Sciences·Marc YesteElisabeth Pinart
Dec 21, 2019·Reproductive Biology and Endocrinology : RB&E·Lukas DedJana Peknicova
Aug 31, 2019·Scientific Reports·Michal ZigoPeter Sutovsky
May 8, 2020·International Journal of Molecular Sciences·Marc YesteElisabeth Pinart
May 7, 2021·Biopreservation and Biobanking·Gul Ipek Gundogan, Abit Aktas

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Methods Mentioned

BETA
ELISA
flow
flow cytometry

Software Mentioned

LUCIA

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