Effect of ethanol on adenosine triphosphate, cytosolic free calcium, and cell injury in rat hepatocytes. Time course and effect of nutritional status

Digestive Diseases and Sciences
A GasbarriniD H Van Thiel

Abstract

The events implicated in the early phases of acute ethanol-induced hepatocyte injury and their relation with the nutritional status of the liver are not clearly defined. We aimed to determine the effect of ethanol on ATP and cytosolic free Ca2+ in hepatocytes isolated from fed or fasted rats. Cell injury was assessed by LDH release and trypan blue uptake, ATP by [31P]NMR spectroscopy, and cytosolic free Ca2+ with aequorin. In control conditions, cells from fasted animals had a lower ATP level (-52%) and a higher cytosolic free Ca2+ (+101%) than did those isolated from fed animals. Ethanol caused a dose-dependent cell injury in both groups. At all ethanol doses, greater, damage occurred when using hepatocytes isolated from fasted rats. In both groups, a dose-dependent decrease in ATP content and a rise in cytosolic free Ca2+ were seen. The magnitude of these changes were significantly greater in the fasted group. In conclusion, these data showed that fasting affects the energy status and cytosolic free calcium level in hepatocytes; ethanol causes a dose-dependent cell injury that occurs in association with a fall in ATP and a rise in cytosolic free Ca2+ levels. The nutritional status of an animals is an important determinant of ...Continue Reading

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
May 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A P Bautista, J J Spitzer
Dec 22, 1988·The New England Journal of Medicine·C S Lieber
Jan 1, 1986·Methods in Enzymology·A B Borle, K W Snowdowne
Aug 1, 1986·The American Journal of Physiology·A B BorleK W Snowdowne
Dec 1, 1988·Cell Calcium·A L NieminenJ J Lemasters
Jan 1, 1987·Methods in Enzymology·H J VogelE M Lohmeier-Vogel
Jan 1, 1986·The International Journal of Biochemistry·E Gillam, L C Ward
Apr 25, 1986·Biochimica Et Biophysica Acta·D J TumaM F Sorrell
Oct 1, 1984·Experimental Cell Research·D L FoxallJ B Mitchell
Jul 28, 1993·Biochimica Et Biophysica Acta·A GasbarriniD Van Thiel
Apr 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K B CamachoD J Tuma
May 8, 1993·Biochimica Et Biophysica Acta·A GasbarriniD H Van Thiel

❮ Previous
Next ❯

Citations

May 20, 1999·Annual Review of Pharmacology and Toxicology·R C Baker, R E Kramer
Sep 11, 2012·Alcoholism, Clinical and Experimental Research·Amy Jacobs-HarperAndrea Romani
Feb 22, 2012·NMR in Biomedicine·Rex E Jeffries, Jeffrey M Macdonald
Sep 16, 2003·Biochimica Et Biophysica Acta·Nancy E RawsonMark I Friedman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.