Effect of ethanol on hydrogen peroxide-induced AMPK phosphorylation

American Journal of Physiology. Gastrointestinal and Liver Physiology
Suthat LiangpunsakulDavid W Crabb


AMP-activated protein kinase (AMPK) responds to oxidative stress. Previous work has shown that ethanol treatment of cultured hepatoma cells and of mice inhibited the activity of AMPK and reduced the amount of AMPK protein. Ethanol generates oxidative stress in the liver. Since AMPK is activated by reactive oxygen species, it seems paradoxical that ethanol would inhibit AMPK in the hepatoma cells. In an attempt to understand the mechanism whereby ethanol inhibits AMPK, we studied the effect of ethanol on AMPK activation by exogenous hydrogen peroxide. The effects of ethanol, hydrogen peroxide, and inhibitors of protein phosphatase 2A (PP2A) [either okadaic acid or PP2A small interference RNA (siRNA)] on AMPK phosphorylation and activity were examined in rat hepatoma cells (H4IIEC3) and HeLa cells. In H4IIEC3 cells, hydrogen peroxide (H(2)O(2), 1 mM) transiently increased the level of phospho-AMPK to 1.5-fold over control (P < 0.05). Similar findings were observed in HeLa cells, which do not express the upstream AMPK kinase, LKB1. H(2)O(2) markedly increased the phosphorylation of LKB1 in H4IIEC3 cells. Ethanol significantly inhibited the phosphorylation of PKC-zeta, LKB1, and AMPK caused by exposure to H(2)O(2). This inhibitory ...Continue Reading


Aug 15, 1991·Analytical Biochemistry·E PaulikG Weber
Jul 1, 1995·The American Journal of Physiology·T EvansJ I Shapiro
May 1, 1993·Metabolism: Clinical and Experimental·M M ZydowoJ Swierczyński
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A GalliDavid W Crabb
Sep 11, 2001·Biochemical and Biophysical Research Communications·S L ChoiJ Ha
Oct 17, 2001·The Journal of Clinical Investigation·G ZhouD E Moller
Apr 29, 2004·Alcoholism, Clinical and Experimental Research·A Leslie MorrowSubhash C Pandey
Mar 23, 2005·Current Opinion in Cell Biology·D Grahame Hardie
Jun 28, 2005·The Journal of Biological Chemistry·Rebecca L HurleyLee A Witters
Dec 31, 2005·Alcoholism, Clinical and Experimental Research·Kengo TomitaToshifumi Hibi
May 19, 2006·Journal of Gastroenterology and Hepatology·Takahiro SasamotoHajime Takikawa
Sep 9, 2006·Journal of Gastroenterology and Hepatology·Dongmei WuXianglin Shi
Dec 7, 2006·The Biochemical Journal·Matthew J SandersDavid Carling
Jan 27, 2007·The Journal of Biological Chemistry·Yong WuMing-Hui Zou
Mar 24, 2007·Science·D Grahame Hardie
Sep 15, 2007·The Journal of Biological Chemistry·Olga GöranssonKei Sakamoto


Jun 10, 2009·Molecular Neurobiology·Jia Luo
Jun 8, 2013·Biochemical and Biophysical Research Communications·Bing WangRui-zen Dong
May 22, 2013·Biochemical Pharmacology·Brice SidPedro Buc Calderon
Dec 24, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Suthat LiangpunsakulDavid W Crabb
Mar 13, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Suthat LiangpunsakulDavid W Crabb
Mar 18, 2009·World Journal of Gastroenterology : WJG·Terrence M Donohue
Jun 16, 2012·Indian Journal of Pharmacology·Karuna Rasineni, Carol A Casey
Apr 9, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Stephen A Stricker
Aug 22, 2015·Expert Review of Gastroenterology & Hepatology·He Joe WangMary Katherine Jung
Jul 24, 2012·Free Radical Biology & Medicine·Defeng WuArthur I Cederbaum
Jul 16, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Rodjawan Supakul, Suthat Liangpunsakul
Jun 26, 2014·Alcoholism, Clinical and Experimental Research·Nsini A UmohGeorges E Haddad
May 7, 2014·Alcoholism, Clinical and Experimental Research·ZhanTao ZhuAmin A Nanji
Oct 30, 2015·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Liu YangJun-Ying Zhou
Nov 9, 2011·Free Radical Biology & Medicine·Alexandre SarreFrançoise Assimacopoulos-Jeannet
Jan 17, 2014·Scientific Reports·Peng ZhouGiles E Duffield
May 10, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stephanie J KingDeclan F McCole
Jul 19, 2020·International Journal of Molecular Sciences·Shengnan Wu, Ming-Hui Zou
Jan 29, 2016·World Journal of Gastroenterology : WJG·Claudia Ress, Susanne Kaser
Dec 14, 2019·World Journal of Gastroenterology : WJG·Xue-Yang ChenChao-Hui Yu
May 14, 2020·Journal of Lipid Research·Jason CorrentiRotonya M Carr
Mar 1, 2020·Medical Hypotheses·David F Wilson, Franz M Matschinsky

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