PMID: 737179Dec 21, 1978Paper

Effect of ethidium bromide on the digestion of chromatin DNA with micrococcal nuclease

Biochimica Et Biophysica Acta
A JerzmanowskiK Toczko

Abstract

Intercalation of ethidium bromide into DNA influences the rate of its digestion with micrococcal nuclease in opposite directions depending on whether it is free DNA or DNA in chromatin. In the case of free DNA the binding of ethidium bromide, starting from a very low concentration, results in the inhibition of the rate of digestion (increasing constantly with the increase of the ethidium bromide/nucleotide ratio). In contrast to free DNA the digestion rate as well as the overall amount of nuclease susceptible DNA is increased upon ethidium bromide binding to chromatin, with maximum enhancement around the saturation of intercalation sites. The saturation of intercalation sites in chromatin leads also to the disappearance of the typical micrococcal nuclease digestion pattern of DNA upon gel electrophoresis. Instead, a random cleavage pattern is observed. These data indicate that partial unwinding of chromatin DNA by ethidium bromide results in unmasking new sites for nuclease action. Interpretation of this finding in terms of the nucleosomal structure of chromatin and the mode of ethidium bromide binding to chromatin DNA indicates that newly unmasked sites are localized within the core particle DNA.

References

Sep 1, 1977·Nature·J T FinchA Klug
Mar 1, 1976·FEBS Letters·A JerzmanowskiK Toczko
Jan 25, 1974·Science·A L Olins, D E Olins
Sep 20, 1974·Nature·M Noll
Jan 1, 1973·Tissue & Cell·I Chet, N Kislev

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Citations

Feb 25, 2009·Nucleic Acids Research·Liliana Krasinska, Daniel Fisher
Apr 16, 1984·Biochemical and Biophysical Research Communications·M J AgostinoT A Beerman
Jun 1, 1984·Biopolymers·G L CoffmanL Yielding
May 1, 1992·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Philippe AlbertIsabelle Lacorre-Arescaldino
Dec 17, 1979·Biochimica Et Biophysica Acta·A JerzmanowskiK Toczko

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