Effect of exercise on concentrations of free amino acids in pools of type I and type II fibres in human muscle with reduced glycogen stores

Acta Physiologica Scandinavica
B Essén-Gustavsson, E Blomstrand

Abstract

A few animal studies have shown that some amino acid concentrations vary between different muscle fibre types. In the present study, amino acid concentrations were measured in separate pools of different fibre types in human skeletal muscle, with reduced glycogen stores, before and after sustained exercise. Five subjects exercised at a submaximal work rate for 60 min and then at a maximal rate for 20 min. Biopsy samples were taken from the vastus lateralis muscle before and after exercise; they were freeze-dried and individual fibres were dissected out. Fragments of these fibres were stained for myosin-adenosine triphosphatase (ATPase) and identified as type I or type II fibres. The concentrations of free amino acids were measured by high performance liquid chromatography (HPLC) in perchloric acid (PCA) extracts containing pools of either type of fibre. After exercise, glycogen was decreased in type I fibres (53%) and in four subjects also in type II fibres. The concentrations of most amino acids were similar in the two fibre types before exercise, but the glutamate, aspartate and arginine levels were 10% higher in type II than in type I fibres. After exercise, the glutamate concentration was decreased by 45% in both fibre type...Continue Reading

References

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Citations

Dec 9, 2004·Current Opinion in Clinical Nutrition and Metabolic Care·Erica P A RuttenNicolaas E P Deutz
Sep 4, 2012·Cellular and Molecular Bioengineering·Yanjun LiGerald M Saidel
Apr 19, 2015·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Alexandre FouréJulien Gondin
May 27, 2011·Equine Veterinary Journal. Supplement·B Essén-GustavssonA Jansson
Apr 6, 2020·European Journal of Applied Physiology·Allyson M SchweitzerIrena A Rebalka
May 4, 2004·Journal of Applied Physiology·Krista R HowarthMartin J Gibala

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