Effect of exercise on gene expression profile in unfractionated peripheral blood leukocytes

Biochemical and Biophysical Research Communications
Seiji NakamuraKenichi Matsubara

Abstract

A 4-h bout of exercise induces immunomodulatory effects. Peripheral blood was withdrawn before, and at 4, 8 and 24h after the start of exercise. RNA from the unfractionated white blood cells was analyzed using Agilent human 44K microarray. The expression profiles were sorted into seven clusters based on their unique time-dependent kinetics. In a separate experiment, cell-specific markers were collected and compared among the members in each cluster. Two clusters were assigned as representing neutrophils, one as NK cells, and another mostly as T cells. Three clusters seemed to be mixtures of several cell types. Extension of this approach to other systems is discussed.

References

Jul 1, 1994·Pharmaceutica acta Helvetiae·D Steinhilber
Dec 17, 2002·Current Opinion in Hematology·Mary C Dinauer
May 18, 2004·Arthritis and Rheumatism·Andrew CrossRobert J Moots
Jun 15, 2004·Journal of Applied Physiology·Peter H ConnollyDan M Cooper
Feb 25, 2005·British Journal of Sports Medicine·J ScharhagW Kindermann
Sep 15, 2005·Cellular Immunology·Adeline Loo Yee KohMichael Glogauer
Feb 14, 2006·Nature Immunology·Kate L JeffreyCharles R Mackay
Apr 28, 2006·Journal of Anatomy·Hechmi ToumiThomas M Best
Feb 17, 2007·Journal of Applied Physiology·Michael Gleeson
Sep 18, 2007·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·Tomoko KawaiKazuhito Rokutan
Nov 22, 2007·The Journal of Biological Chemistry·Sarah A BissonnetteMichael B Yaffe
Feb 1, 1979·IEEE Transactions on Pattern Analysis and Machine Intelligence·D L Davies, D W Bouldin

❮ Previous
Next ❯

Citations

Jan 7, 2014·PloS One·Stefano CapomaccioKatia Cappelli
Sep 21, 2010·Biochemical and Biophysical Research Communications·Shruta S DadarkarMuralidhara Padigaru
Nov 12, 2013·International Journal of Experimental Pathology·Neide M MoreiraSilvana M d Araujo
Jul 21, 2015·Stem Cells and Development·Lindolfo da Silva MeirellesDimas Tadeu Covas
Aug 2, 2011·International Journal of Radiation Biology·Sunirmal Paul, Sally A Amundson
Oct 28, 2011·Drug Testing and Analysis·Elmo W I NeubergerPerikles Simon
Jun 11, 2021·Nature Communications·David AmarEuan A Ashley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.