Effect of exogenous extracellular polysaccharides on the desiccation and freezing tolerance of rock-inhabiting phototrophic microorganisms

FEMS Microbiology Ecology
Emily J Knowles, Richard W Castenholz

Abstract

Two major stresses that threaten rock-inhabiting microbial communities are desiccation and freezing; both result in a loss of liquid water in the cells. The mechanisms necessary to tolerate these extremes may be similar, but are not well understood. In both cases extracellular polysaccharides (EPS) seem to play an important role. This study examines whether the EPS released by a rock-inhabiting phototroph can have a protective effect on other members of similar and neighboring microbial communities. This interaction was modeled by adding EPS isolated from the cryptoendolithic cyanobacterium Nostoc sp. to cells of the cryptoendolithic green alga Chlorella sp. and to cells of the epilithic cyanobacterium Chroococcidiopsis sp. The cells were then subjected to desiccation and freezing and the survival rates were determined by vital staining, using membrane integrity as a measure of viability. The results clearly demonstrate the importance of exogenous EPS in the desiccation tolerance of both species, while mixed results were found for the freezing trials.

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Citations

Mar 12, 2011·Die Naturwissenschaften·Francesco Canganella, Juergen Wiegel
May 20, 2011·Colloids and Surfaces. B, Biointerfaces·Hans-Curt Flemming
Oct 24, 2015·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yohei ShimuraMasanobu Kawachi
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May 12, 2012·Journal of Microbiological Methods·Andreas NockerFrank Schuren
Mar 21, 2017·Nature Reviews. Microbiology·Pedro H LebreDon A Cowan
Mar 6, 2018·FEMS Microbiology Ecology·Nathan A M ChrismasPatricia Sánchez-Baracaldo
Mar 3, 2020·Frontiers in Microbiology·Pervaiz AliFeng Chen
Mar 15, 2020·Applied Microbiology and Biotechnology·Vincent Robert Guy Greffe, Jan Michiels
Sep 8, 2021·Frontiers in Microbiology·Aparna BanerjeeRajesh K Sani

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