Effect of exposure of rabbit hepatocytes to sulfur-containing anthelmintics (oxfendazole and fenbendazole) on cytochrome P4501A1 expression

Toxicology in Vitro : an International Journal Published in Association with BIBRA
C Gleizes-EscalaP Galtier

Abstract

The expression of cytochrome P4501A1 and 1A2 was investigated in rabbit hepatocytes maintained in primary cultures for 96 hr in the absence or presence of 100 mum of the benzimidazole anthelmintics oxfendazole or fenbendazole. Total cytochrome P-450, ethoxyresorufin O-deethylase and acetanilide hydroxylase activities were significantly increased in cell cultures receiving benzimidazoles. These increases were more marked after exposure of cultured hepatocytes to oxfendazole (OFZ) than to fenbendazole (FBZ). Western and Northern blot analysis of microsomes and RNA prepared from these cultures revealed increased levels of both protein and specific mRNA for P4501A1. The inhibition of these inductions in the presence of actinomycin D suggests a transcriptional way of activation of this gene. The ability of OFZ to bind to the Ah receptor has been examined. Data obtained from competition experiments with dioxin demonstrated that OFZ and other compounds in the benzimidazole series are not ligand of the Ah receptor. From saturation experiments and Scatchard plot analysis, rabbit hepatocyte Ah receptor (K(d) = 10.6 nm) seems to belong, as does the human Ah receptor, to a low-affinity category. Different induction rates obtained with seve...Continue Reading

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Citations

Apr 28, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Roger J PriceBrian G Lake
Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C EeckhoutteM Dacasto
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Michael S Denison, Scott R Nagy
Jun 13, 2003·Research in Veterinary Science·V BaliharováJ Fink-Gremmels
Dec 16, 2003·Research in Veterinary Science·J VelíkL Skálová
Apr 21, 2004·Journal of Veterinary Pharmacology and Therapeutics·V BaliharováL Skálová
Jun 1, 2005·Research in Veterinary Science·M SavlíkL Skálová
Jan 4, 2015·Chemico-biological Interactions·Lucie StuchlíkováLenka Skálová
Dec 16, 1998·Toxicology and Applied Pharmacology·M S DenisonM H Ziccardi
Jun 13, 2012·The Journal of Toxicological Sciences·Hitomi HayashiKunitoshi Mitsumori

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Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.