Effect of externally applied electrostatic fields on the surface topography of ceramide-enriched domains in mixed monolayers with sphingomyelin

Biophysical Chemistry
Natalia Wilke, Bruno Maggio

Abstract

Lipid and protein molecules anisotropically oriented at a hydrocarbon-aqueous interface configure a dynamic array of self-organized molecular dipoles. Electrostatic fields applied to lipid monolayers have been shown to induce in-plane migration of domains or phase separation in a homogeneous system. In this work, we have investigated the effect of externally applied electrostatic fields on the distribution of the condensed ceramide-enriched domains in mixed monolayers with sphingomyelin. In these monolayers, the lipids segregate in different phases at all pressures. This allows analyzing by epifluorescence microscopy the effect of the electrostatic field at all lateral pressure because coexistence of lipid domains in condensed state are always present. Our observations indicate that a positive potential applied to an electrode placed over the monolayer promotes a repulsion of the ceramide-enriched domains which is rather insensitive to the film composition, depends inversely on the lateral pressure and exhibits threshold dependence on the in-plane elasticity.

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Citations

Apr 27, 2011·Chemical Communications : Chem Comm·Frank J ZendejasDarryl Y Sasaki
Feb 12, 2014·Progress in Lipid Research·Bruno M CastroLiana C Silva
Sep 18, 2012·Chemistry and Physics of Lipids·Andrea Alejandra Bischof, Natalia Wilke
Apr 15, 2014·Advances in Colloid and Interface Science·Małgorzata JurakEmil Chibowski
Jan 14, 2010·Biochimica Et Biophysica Acta·Maria Laura FananiRafael Gustavo Oliveira
Jan 26, 2012·Chemistry and Physics of Lipids·Franco Vega MercadoNatalia Wilke
Jun 22, 2016·European Biophysics Journal : EBJ·Petar EftimovGeorgi As Georgiev
Jan 31, 2015·Soft Matter·Andrea Alejandra BischofNatalia Wilke
Dec 1, 2011·Biophysics Reviews·Natalia Wilke, Bruno Maggio
Apr 13, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·N WilkeB Maggio
Dec 19, 2013·The Journal of Physical Chemistry. B·Benjamín CarusoNatalia Wilke

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