Effect of Extracellular Matrix Composition on the Expression of Glutathione S-transferase Isoenzymes in Organotypical Hepatocyte Cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S BekenV Rogiers

Abstract

Collagen gel sandwich and immobilization cultures of hepatocytes, using hydrated collagen type I as extracellular matrix (ECM), have been proposed as long-term in vitro models in pharmaco-toxicology. The in vivo ECM composition in the space of Disse is, however, much more complex. As a differentiated hepatocyte phenotype is thought to be highly dependent on ECM composition and biophysical characteristics, we modulated the ECM to mimic the in vivo situation. Moreover, commercially available collagen type I (Boehringer-Ingelheim) was compared to the one prepared in the laboratory from rat tails. ECM composition had no effect on albumin secretion or hepatocyte morphology in both collagen gel sandwich and immobilization cultures. Total, Alpha and Mu class GST activities in organotypical cultures with a complex or a simple collagen type I ECM were similar. The Pi class GST activity increased as a function of culture time in all culture models. Thus, mimicking the in vivo composition of the ECM did not improve the changes in GST expression that were observed in simple collagen gel cultures. The collagen type I matrix is therefore assumed to confer sufficient protection to help the hepatocytes to maintain their differentiated phenotyp...Continue Reading

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Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BekenV Rogiers
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K de SmetV Rogiers
Dec 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BekenV Rogiers

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Citations

Jan 29, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lysiane RichertE LeCluyse
Jan 23, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Myung Hee KimNam-Joon Cho
Apr 27, 2016·International Journal of Pharmaceutics·Christian Kölln, Stephan Reichl

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