Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices.

Journal of Veterinary Science
G GangaR L Sharma

Abstract

The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37 degrees C, and centrifuging the supernatant at 12,000 g at 4 degrees C for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days. Hemograms of both groups were studied initially and on days 0, 2, 4, 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant (p < 0.01) declines in the values of hemoglobin, hematocrit, and total erythrocyte count were observed without significant (p > 0.05) changes in the values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, or mean corpuscular volume in group A. Thus, we conclude that Fg-ESA induces normocytic normochromic anemia in rats.

References

Apr 1, 1971·Journal of Comparative Pathology·J D Dargie, W Mulligan
Jan 1, 1981·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·D PerrissoudM F Maignan
Oct 1, 1996·Veterinary Immunology and Immunopathology·D T MasihJ M Casado

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Citations

Jun 2, 2012·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·R L SharmaM B Thilagar
Sep 23, 2008·Acta Tropica·M A ValeroS Mas-Coma
Mar 14, 2019·Toxicology and Industrial Health·Samah Saleh El-HadidyAbdel Hamied Abdel Hamied

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