PMID: 1201762Nov 21, 1975Paper

Effect of fasting on two postheparin plasma triglyceride lipases and triglyceride removal in obese subjects

European Journal of Clinical Investigation
J K HuttunenM Ohta

Abstract

A new method was used for selective measurement of lipoprotein lipase and hepatic lipase in human postheparin plasma. Hepatic lipase was assayed in 1.0 M NaCl withour addition of serum, and the activity of lipoprotein lipase was determined in 0.1 M NaCl after immunoprecipitation of hepatic lipase with specific antiserum. The activity of both these enzymes and the total lipolytic activity were measured in plasma samples taken during a 4-h infusion of heparin. Each of the activities was related to basal serum triglyceride concentration and to the fractional removal constant (K) of Intralipid in 13 obese subjects before and after prolonged fasting. During a normal isocaloric diet the lipolytic activities showed a biphasic response to heparin infusion in all subjects. A peak activity was reached within 30 minutes ("early response") and thereafter the lipase activities decreased to a constant level maintained during the rest of the heparin infusion ("late response"). The early response of lipoprotein lipase showed a significant inverse correlation with the basal serum triglyceride level (r = -0.85) and a significant positive correlation with the fractional removal rate of Intralipid (r = 0.84). The late response of lipoprotein lipas...Continue Reading

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Citations

May 1, 1981·Kidney International·M K ChanJ F Moorhead
Jan 1, 1985·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·B VessbyH Lithell
Mar 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J ZimmermanY Stein
Jan 1, 1985·Upsala Journal of Medical Sciences·H LithellB Vessby

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