PMID: 7371116Apr 1, 1980Paper

Effect of fatty acids on physical properties of microsomes from isolated perfused rat liver

Chemistry and Physics of Lipids
F Schroeder, E H Goh

Abstract

A computer-centered spectrofluorimeter was used to examine the physicochemical properties of hepatic microsomes and microsomal lipids obtained from isolated rat livers perfused with medium containing palmitate or oleate. The fatty acid composition and degree of unsaturation of the liver microsomal lipids reflected that the fatty acid present in the perfusate. The absorption corrected fluorescence, relative fluorescence efficiency, polarization, and fluorescence anisotropy of several fluorescent probe molecules were measured to determine if their different microenvironments may be altered by the type of fatty acid infused. The probe molecules beta--parinaric acid and 1,6-diphenyl-1,3,5-hexatriene had higher values for each of these parameters when incorporated into microsomes obtained from livers perfused with a medium containing palmitate than with oleate. The same parameters measured for cholesta-5,7,9(11)-trien-3 beta-ol and N-phenyl-1-naphthylamine were not altered. These differences appeared to be primarily due to alterations in microviscosity of the probe microenvironments since the rotational correlation time of 1,6-diphenyl-1,3,5-hexatriene was 25% lower in the microsomes from livers perfused with oleate as compared to l...Continue Reading

References

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Citations

Nov 1, 1982·Quarterly Reviews of Biophysics·R A Klein
May 16, 1983·European Journal of Biochemistry·F Schroeder, C Soler-Argilaga
May 16, 1983·European Journal of Biochemistry·F Schroeder
Sep 9, 2006·Journal of Lipid Research·Nica M BorradaileJean E Schaffer
Oct 23, 2012·The Journal of Biological Chemistry·Elena DeliuEugen Brailoiu
Jun 23, 2016·Journal of Lipid Research·Bharat Jaishy, E Dale Abel
Feb 7, 1983·Biochimica Et Biophysica Acta·G R GourleyG B Odell

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