Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats

Neuroscience Letters
Fausto Pierdoná GuzenJeferson Sousa Cavalcante

Abstract

Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n=10), or fragment of the sciatic nerve (Nerve group, n=10), or fragment of the sciatic nerve to which FGF-2 (Nerve+FGF-2 group, n=10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to comp...Continue Reading

References

Jun 1, 1993·Journal of Neurology, Neurosurgery, and Psychiatry·T P CarlstedtI A Nilsson-Remahl
Dec 1, 1995·The European Journal of Neuroscience·R R JiT Hökfelt
Jun 17, 2006·Annual Review of Neuroscience·Sophie Pezet, Stephen B McMahon
Jul 17, 2012·Molecular Neurodegeneration·Victor Tulio Ribeiro-ResendeRosalia Mendez-Otero
Mar 19, 2015·Journal of Molecular Cell Biology·Hui LiuXu Zhang

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