PMID: 6986532Feb 1, 1980Paper

Effect of fibroblast growth factor on cultured fetal rat calvaria

Metabolism: Clinical and Experimental
E Canalis, L G Raisz

Abstract

Normal rat or human serum causes a greater incorporation of 3H-proline into bone collagenase digestible protein (CDP) and noncollagen protein (NCP) than does serum from hypophysectomized animals or hypopituitary humans. In the present study, we have tested fibroblast growth factor (FGF), a peptide isolated from bovine pituitary glands that has been shown to stimulate RNA and DNA synthesis in various mesodermal cells, for its effects on cultured fetal rat calvaria. The major effect of FGF appeared to be a stimulation of periosteal fibroblastic cell proliferation. Incorporation of 3H-thymidine into DNA was increased at concentrations of 10--1000 ng/ml; the effect appeared after 12 hr, was sustained for 96 hr, and could not be ascribed to an effect on 3H-thymidine uptake. Total DNA content was increased and histologic sections showed an increase in the number of mitoses in periosteal fibroblasts after colemid arrest. These effects were accompanied by an increase in the uptake and incorporation of 3H-uridine, a decrease in the incorporation of labeled proline into CDP, and a small and variable increase in the incorporation of proline into NCP. Cortisol opposed the effects of FGF on 3H-thymidine and 3H-uridine incorporation. Insulin...Continue Reading

References

Jan 1, 1975·Advances in Metabolic Disorders·D GospodarowiczK Benirschke
Apr 1, 1977·The Journal of Clinical Endocrinology and Metabolism·D GospodarowiczN D Owashi
Dec 14, 1978·Biochemical and Biophysical Research Communications·A H Tashjian, L Levine
Apr 1, 1979·Endocrinology·E Canalis, L G Raisz
Mar 1, 1979·Endocrinology·J W DietrichL G Raisz
Oct 1, 1977·Metabolism: Clinical and Experimental·E M CanalisL G Raisz
Aug 1, 1976·The Journal of Cell Biology·D GospodarowiczJ Lindstrom
Jan 1, 1975·Advances in Metabolic Disorders·B Westermark, A Wasteson
Aug 1, 1975·The Journal of Cell Biology·D Gospodarowicz, J S Moran
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P S RudlandD Gospodarowicz
Jan 1, 1974·Calcified Tissue Research·P J Bingham, L G Raisz
May 26, 1972·Biochemical and Biophysical Research Communications·L LevineA H Tashjian

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Citations

Aug 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S PitaruN Savion
Jul 7, 1983·The New England Journal of Medicine·L G Raisz, B E Kream
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Centrella, E Canalis
Feb 1, 1990·The Proceedings of the Nutrition Society·I P Braidman
Jul 21, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Guomin OuGloria Gronowicz
Apr 8, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Joseph Caverzasio, Cyril Thouverey
May 1, 1988·The Journal of Clinical Investigation·E CanalisT McCarthy
Jul 2, 2014·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Alberto Fernández-JaénSara López-Martín
Mar 30, 1988·Biochemical and Biophysical Research Communications·C A FrolikD C Williams
May 12, 2006·Periodontology 2000·Francis J HughesGianluca Martuscelli
Jan 1, 1989·Connective Tissue Research·S B RodanG A Rodan
Jun 28, 1985·Biochemical and Biophysical Research Communications·A van der PlasP J Nijweide
Oct 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B KrempienR Büchele
Nov 3, 1980·FEBS Letters·M MagnaniG Fornaini

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