Effect of flavin compounds on glutathione reductase activity: in vivo and in vitro studies

The Journal of Clinical Investigation
E Beutler

Abstract

Increases or decreases of red cell glutathione reductase (GR) have been described in connection with many clinical abnormalities. We find that GR activity as measured in hemolysates represents only a portion of the available GR activity. The addition of small amounts of flavin adenine dinucleotide (FAD), but not of flavin mononucleotide or riboflavin, activates the GR of hemolysates. 1 muM FAD results in a maximal activation within 10 min; gradually increasing activation occurs at much lower, for example, 20 mmuM FAD concentrations. Once FAD has activated GR, dilution or dialysis does not reverse activation of the enzyme. Activation of GR by FAD can be inhibited by adenosine triphosphate (ATP), and to a lesser extent by adenosine diphosphate (ADP) and adenosine monophosphate (AMP), if these adenine nucleotides are added before the addition of FAD, but only to a slight extent if FAD is added before the adenine nucleotides. The addition of FAD to GR does not alter its electrophoretic mobility but produces intensification of the bands. The administration of 5 mg of riboflavin daily produces marked stimulation of red cell GR activity within only 2 days. After cessation of riboflavin administration, the GR activity again begins to f...Continue Reading

References

Mar 25, 1968·Biochimica Et Biophysica Acta·K G BlumeG W Löhr
May 24, 1968·Deutsche medizinische Wochenschrift·K HaydukH D Waller
Apr 1, 1953·The Journal of Pathology and Bacteriology·H FOY, A KONDI
Sep 1, 1959·The Journal of Clinical Investigation·E BEUTLER

❮ Previous
Next ❯

Citations

Nov 1, 1975·Arthritis and Rheumatism·W N KelleyM B Van der Weyden
Jan 1, 1972·Naunyn-Schmiedeberg's Archives of Pharmacology·R SchroeterW Vogt
Oct 1, 1990·Bulletin of Environmental Contamination and Toxicology·H SetoT Kanoh
Jul 1, 1974·Bulletin of Environmental Contamination and Toxicology·H M Mykkanen, H E Ganther
Jul 31, 1975·Molecular and Cellular Biochemistry·J van Eys
Mar 1, 1995·Metabolic Brain Disease·B Wood, J Currie
Mar 1, 1980·Irish Journal of Medical Science·S C Vir, A H Love
Jul 14, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y Yawata, K R Tanaka
Apr 1, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Ramachandran, G Y Iyer
Dec 2, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·H ArnoldY Najean
May 1, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·G E StaalJ F Koster
Jun 2, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·R K HoornD Westerink
Sep 6, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·K G BlumeG W Löhr
Mar 1, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Shinohara, K R Tanaka
Dec 23, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·T R HardwellM Whittaker
Apr 28, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·J OkudaT Hayazaki
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·V A ReddyA A Paul
Jan 1, 1985·Mechanisms of Ageing and Development·G A Hazelton, C A Lang
Mar 15, 1990·Mechanisms of Ageing and Development·K Spodaryk
Nov 1, 1992·Mechanisms of Ageing and Development·M A Rodriguez-Martinez, A Ruiz-Torres
Oct 1, 1985·Archives of Gerontology and Geriatrics·N TakeuchiK Uchida
Mar 1, 1985·Prostaglandins, Leukotrienes, and Medicine·N J PelliccioneJ Pinto
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·T SuzukiM Suzuki
Oct 1, 1980·Biochimica Et Biophysica Acta·J E Clements, B B Anderson
Nov 15, 1973·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P G LankischW Vogt
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T SuzukiM Suzuki
Nov 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M KurataN S Agar
Aug 30, 1994·International Journal of Radiation Oncology, Biology, Physics·R WollmanZ Fuks
Jan 15, 1996·International Journal of Radiation Oncology, Biology, Physics·M S KhilJ H Kim
Nov 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V HernoF Erb
Jan 1, 1988·Free Radical Biology & Medicine·D V Godin, S A Wohaieb
Jan 1, 1991·Free Radical Biology & Medicine·C K Chow
Jul 1, 1993·Free Radical Biology & Medicine·A ShuklaR C Srimal
Jan 1, 1996·Free Radical Biology & Medicine·J M MayC E Cobb
Jan 1, 1995·European Journal of Cancer. Part B, Oral Oncology·K KrishnaswamyG A Reddy
Nov 26, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bondada Andallu, N Ch Varadacharyulu
Nov 24, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R AgarwalG S Shukla
Jan 21, 2000·Free Radical Biology & Medicine·T Nomura, K Yamaoka
Oct 4, 2002·Nutrition·Yun-Zhong FangGuoyao Wu
Jul 31, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·O HusseinM Aviram

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.