Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels

PloS One
Robin SimsaPer Fogelstrand

Abstract

Decellularization of blood vessels is a promising approach to generate native biomaterials for replacement of diseased vessels. The decellularization process affects the mechanical properties of the vascular graft and thus can have a negative impact for in vivo functionality. The aim of this study was to determine how detergents under different fluid dynamics affects decellularization efficacy and mechanical properties of the vascular graft. We applied a protocol utilizing 1% TritonX, 1% Tributyl phosphate (TnBP) and DNase on porcine vena cava. The detergents were applied to the vessels under different conditions; static, agitation and perfusion with 3 different perfusion rates (25, 100 and 400 mL/min). The decellularized grafts were analyzed with histological, immunohistochemical and mechanical tests. We found that decellularization efficacy was equal in all groups, however the luminal ultrastructure of the static group showed remnant cell debris and the 400 mL/min perfusion group showed local damage and tearing of the luminal surface. The mechanical stiffness and maximum tensile strength were not influenced by the detergent application method. In conclusion, our results indicate that agitation or low-velocity perfusion with d...Continue Reading

References

Mar 1, 1989·The Histochemical Journal·C Farquharson, S P Robins
May 1, 1997·The International Journal of Biochemistry & Cell Biology·M A Horton
Feb 24, 2001·Yonsei Medical Journal·R M Nerem
Jun 26, 2004·Journal of Vascular Surgery·Patrick J SchanerPaul J Dimuzio
Jan 1, 2008·The Open Orthopaedics Journal·Roel G M BreulsTheo H Smit
Dec 19, 2009·Regenerative Medicine·Swathi Ravi, Elliot L Chaikof
Feb 8, 2011·Biomaterials·Peter M CrapoStephen F Badylak
Mar 23, 2011·Annual Review of Biomedical Engineering·Stephen F BadylakKorkut Uygun
Mar 31, 2012·The International Journal of Artificial Organs·Alessandro F PellegataSara Mantero
Nov 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Krishna MadhavanWei Tan
Jun 1, 2013·Biomaterials·Paul E BourgineIvan Martin
Dec 3, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Bryan N Brown, Stephen F Badylak
Apr 22, 2014·Interface Focus·Ellen M GreenC Peter Winlove
Jan 30, 2015·Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·Cordula FelixRobert-Jan M van Geuns
Jan 30, 2015·Vascular Health and Risk Management·Neelima Thottappillil, Prabha D Nair
Oct 4, 2015·Acta Biomaterialia·Karl Heinrich SchneiderAndreas Herbert Teuschl
Oct 9, 2015·Tissue Engineering. Part B, Reviews·Samand Pashneh-TalaFrederik Claeyssens
Oct 16, 2015·Biotechnology Letters·Guifang Gao, Xiaofeng Cui
Nov 13, 2015·Cells, Tissues, Organs·Alessandro F PellegataSara Mantero
Jan 17, 2016·Journal of Cellular Physiology·Kelsey D SackMatthew A Nugent
Mar 20, 2016·Journal of Vascular Surgery. Venous and Lymphatic Disorders·Vijay Kumar KunaJørgen J Jørgensen
Aug 6, 2016·Biomechanics and Modeling in Mechanobiology·Jeffrey M MattsonYanhang Zhang
Oct 4, 2016·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Thorsten M SeylerPatrick W Whitlock
Jan 28, 2017·Nature Communications·Mitasha BharadwajDaniel J Müller
Aug 5, 2017·Materials·Xiaohong WangShuling Bai
Nov 5, 2017·Journal of the Mechanical Behavior of Biomedical Materials·Yunjie WangYanhang Zhang
Aug 14, 2018·Journal of Visualized Experiments : JoVE·Vijay Kumar KunaSuchitra Sumitran-Holgersson
Jul 30, 2019·Journal of Current Glaucoma Practice·Rick E Bendel, Michael T Patterson

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Citations

Nov 25, 2020·Tissue Engineering. Part a·Megan KimicataJohn P Fisher
Feb 16, 2021·Frontiers in Cardiovascular Medicine·Bruna B J LealPatricia Pranke

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Methods Mentioned

BETA
biopsies
Fluorescence
Assay
scanning
biopsy

Software Mentioned

ImageJ
GraphPad Prism

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