Effect of four lichen acids isolated from Hypogymnia physodes on viability of rat thymocytes

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Voja PavlovicGordana S Stojanović

Abstract

Four lichen acids, physodalic acid (F1), physodic acid (F2), 3-hydroxyphysodic acid (F3), and isophysodic acid (F4), were isolated from Hypogymnia physodes methanol extract using preparative reversed-phase high performance liquid chromatography and their structures were determined by UV, MS, (1)H NMR and (13)C NMR. This is the first report on the isolation of F4 from H. physodes. Isolated rat thymocytes were cultivated with increasing F1-F4 concentrations (0.1, 1, 10μg/well) and proliferative activity, viability, ROS (reactive oxygen species) production and MMP (mitochondrial membrane potential) disturbances were evaluated. Obtained results show significantly decreased thymocytes proliferation was observed when cells were treated with F1 (1μg, p<0.05; 10μg; p<0.001), F2 (10μg, p<0.05) and F3 compound (10μg, p<0.05). Significantly increased cytotoxicity was detected when cells were incubated with F1 (1μg, p<0.05; 10μg, p<0.01), F2 (10μg, p<0.05) and F3 compound (10μg, p<0.001). Increased H2DCF-DA fluorescence intensity, when cells were treated with F1 (1μg, p<0.001; 1μg, p<0.01; 10μg, p<0.001) and F2 (1μg, p<0.05; 10μg, p<0.01) compound, indicating the increase of intracellular ROS production. Simultaneously, increased ROS level...Continue Reading

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Citations

Sep 16, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pollyanna A S WhiteMarcio R V Santos
Jan 15, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Voja PavlovicJelena Jovanovic
Apr 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Maonian XuSesselja Omarsdottir
Apr 7, 2016·Pharmaceutical Biology·Elżbieta Studzińska-SrokaWiesława Bylka
Dec 17, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jinyao ChenLishi Zhang
Apr 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mônica Cristina Barroso MartinsNicácio Henrique da Silva
Mar 25, 2018·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Bugrahan EmsenAli Aslan
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna HawryłŁukasz Komsta
Nov 26, 2020·The Science of the Total Environment·R Sagaya JansiAmal Al-Tamimi
May 26, 2021·Planta medica·Isabel Ureña-VacasM Pilar Gómez-Serranillos

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