Abstract
Four lichen acids, physodalic acid (F1), physodic acid (F2), 3-hydroxyphysodic acid (F3), and isophysodic acid (F4), were isolated from Hypogymnia physodes methanol extract using preparative reversed-phase high performance liquid chromatography and their structures were determined by UV, MS, (1)H NMR and (13)C NMR. This is the first report on the isolation of F4 from H. physodes. Isolated rat thymocytes were cultivated with increasing F1-F4 concentrations (0.1, 1, 10μg/well) and proliferative activity, viability, ROS (reactive oxygen species) production and MMP (mitochondrial membrane potential) disturbances were evaluated. Obtained results show significantly decreased thymocytes proliferation was observed when cells were treated with F1 (1μg, p<0.05; 10μg; p<0.001), F2 (10μg, p<0.05) and F3 compound (10μg, p<0.05). Significantly increased cytotoxicity was detected when cells were incubated with F1 (1μg, p<0.05; 10μg, p<0.01), F2 (10μg, p<0.05) and F3 compound (10μg, p<0.001). Increased H2DCF-DA fluorescence intensity, when cells were treated with F1 (1μg, p<0.001; 1μg, p<0.01; 10μg, p<0.001) and F2 (1μg, p<0.05; 10μg, p<0.01) compound, indicating the increase of intracellular ROS production. Simultaneously, increased ROS level...Continue Reading
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