Effect of frame rate capture frequency on sperm kinematic parameters and subpopulation structure definition in boars, analysed with a CASA-Mot system

Reproduction in Domestic Animals = Zuchthygiene
Anthony ValverdeCarles Soler

Abstract

Motility is the most widely used indicator of sperm quality. Computer-Assisted Semen Analysis (CASA) allows the objective evaluation of sperm motility parameters. CASA technology is a common tool to predict semen doses in farm animal reproduction. The kinds of video cameras used until now for image acquisition have presented limited frame rates (FR), which have a negative influence on the quality of the obtained data. The aim of the present work was to define the optimal frame rate for a correct evaluation of boar sperm motility and its subpopulation structure. Eighteen ejaculates from nine mature boars of the Pietrain breed were used. Using the ISAS® v1 CASA-Mot system, with a video camera working up to 200 Hz, six FRs (25, 50, 75, 100, 150 and 200 fps) were compared. ISAS® D4C20 counting chambers, warmed to 37°C, were used. FR affected all the kinematic parameters, with curvilinear velocity (VCL) and BCF the most sensitive ones. All the parameters showed differences among animals. Non-linear correlation showed the asymptotic level for VCL at 212 fps, being the highest FR for all the parameters. For future studies based just on progressive motility, almost 100 fps FR for 0.5 s must be used, while when kinematics must be consid...Continue Reading

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Citations

Mar 11, 2021·PloS One·Anthony ValverdeEduardo R S Roldan
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Vinicio BarqueroAnthony Valverde
Apr 22, 2021·Physiological Reviews·Maria Eugenia Teves, Eduardo R S Roldan
May 16, 2021·Andrologia·Ndivhuho Beauty TakalaniChinyerum Sylvia Opuwari
Jun 12, 2021·Frontiers in Veterinary Science·Sabrina GacemJordi Miró

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