PMID: 7216459Jan 1, 1981

Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production, and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture

Infection and Immunity
L N HardyA J Wicken

Abstract

Streptococcus mutans Ingbritt was grown in a chemostat at destined dilution rates in either 0.5% fructose or 0.5% sorbitol and at destined pH values in 0.5% fructose. The yield of cells was affected by the carbohydrate source, as well as by the pH, with the lowest yield being at pH 5.5 in 0.5% fructose. Fructose-grown cells showed greater susceptibility to lysis by a muramidase than the corresponding glucose-grown cells, but there were no marked differences in the lytic susceptibilities of the corresponding cell wall preparations or in the serological reactivities of wall lysates with antiserum to S. mutans Ingbritt. The greatest amounts of cellular lipoteichoic acid were obtained at high dilution rates in both fructose and sorbitol, as well as at high pH values in fructose. The greatest amounts of extracellular lipoteichoic acid were found at low dilution rates, as estimated by rocket immunoelectrophoresis and also by hemagglutination. Three major extracellular protein components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the effects of growth conditions on these components were determined. Results for batch-grown cultures showed that there was genotypic variation in the susceptibility of ...Continue Reading

Citations

Jan 1, 1985·Journal of Periodontal Research·W J PerosZ Skobe
Apr 1, 1997·Advances in Dental Research·R A BurneJ E Penders
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, James Baddiley
Aug 15, 1994·FEMS Microbiology Letters·R A Burne, J E Penders
Oct 16, 2014·Journal of Oral Microbiology·Zachary D MoyeR A Burne
Mar 13, 2015·Frontiers in Cellular and Infection Microbiology·Marlise I KleinHyun Koo
Feb 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·H MiörnerG Kronvall
Mar 13, 1998·Journal of Dental Research·R A Burne

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