PMID: 8982265Dec 13, 1996Paper

Effect of fumonisin B1 on phosphatidylethanolamine biosynthesis in Chinese hamster ovary cells

Biochimica Et Biophysica Acta
K BadianiN D Ridgway

Abstract

Fumonisin B1 has been shown to inhibit dihydroceramide synthesis and elevate cellular sphinganine levels in several cultured cell lines. In Chinese hamster ovary (CHO)-K1 cells, 20 microM fumonisin B1 inhibited sphingomyelin synthesis by 75% after 5 h, but stimulated [3H]serine incorporation into PtdEtn by 5- to 7-fold. Fumonisin caused a 10-20% increase in [3H]serine labelling of PtdSer. While fumonisin (20 microM) caused sustained inhibition of sphingomyelin synthesis, PtdEtn labelling peaked at 7-fold above controls at 12 h and declined to 4-fold by 24 h. Fumonisin treatment for 12 h increased the in vitro activity of PtdSer synthase by 62% and inhibited PtdSer decarboxylase by 35%, suggesting that increased PtdEtn labelling by [3H]serine is not by this pathway. An ethanolamine 'trap' experiment was performed to assess the contribution of phosphoethanolamine from sphinganine degradation for PtdEtn labelling. Stimulation of [3H]serine incorporation into PtdEtn by fumonisin could be reduced by 60% with the inclusion of 50 microM unlabelled ethanolamine in the culture medium. The ethanolamine-mediated reduction in [3H]serine incorporation into PtdEtn was accompanied by 4-fold increase in cellular [3H]phosphoethanolamine. In con...Continue Reading

References

Jun 27, 1973·Molecular and Cellular Biochemistry·W Stoffel
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D R Voelker
Apr 28, 1995·Biochimica Et Biophysica Acta·N D Ridgway, D L Merriam
Jun 2, 1995·The Journal of Biological Chemistry·W I WuG M Carman
Jul 21, 2006·The Journal of Physical Chemistry. B·Martin MuchaPavel Jungwirth

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Citations

Mar 13, 2002·Toxicology Letters·Maria C FerranteAntonia Lucisano
May 22, 2001·Environmental Health Perspectives·R T RileyA H Merrill
Sep 2, 1998·International Journal of Cancer. Journal International Du Cancer·A M DiPietrantonioJ M Wu

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