Effect of furosemide infusion on renal hemodynamics and angiogenesis gene expression in acute renal ischemia/reperfusion

Renal Failure
Natarajan Aravindan, Andrew Shaw

Abstract

Loop diuretics are known to affect renal hemodynamics and possibly gene transcription, but the specific effect of furosemide on renal angiogenesis gene expression after acute ischemia is not known. We utilized an acute renal failure model in rats to test the hypothesis that furosemide improves renal hemodynamics and alters the transcriptional signature of acute ischemic nephropathy. Twenty-four male Sprague-Dawley rats were anesthetized by the intraperitoneal administration of 50 mg/kg urethane. Animals were divided into four groups (n = 6 each): (1) sham-operated group infused with saline; (2) sham-operated group infused with 30 microg/kg/hr furosemide (equivalent to a human dosage of 2 mg/hr); (3) unilateral renal ischemia (1 hr, left renal artery cross-clamping) followed by 6 hr of reperfusion; and (4) renal ischemia/ reperfusion (I/R) with furosemide. Renal artery blood flow (RBF), renal cortical perfusion (RCP), and renal corticomedullary tissue oxygen tension (PO2) were recorded throughout. Following 6 hr of reperfusion, left kidney RNA was used to probe microarrays. Gene expression was measured as percent positive control and confirmed using reverse transcriptase polymerase chain reaction. Physiologic data were analyzed ...Continue Reading

References

Nov 24, 1973·British Medical Journal·F CantarovichJ Tizado
Nov 22, 1966·Annals of the New York Academy of Sciences·F C Reubi
Dec 1, 1967·Circulation Research·A G BirtchA C Barger
Oct 1, 1984·Kidney International·M BrezisF H Epstein
Jul 1, 1995·Cleveland Clinic Journal of Medicine·F Cosentino
May 30, 1996·The New England Journal of Medicine·R ThadhaniJ V Bonventre
Dec 15, 1996·Circulation·P V PrasadF H Epstein
Jul 10, 1998·Cell·S E CrawfordN Bouck
Feb 23, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C Justicia, A M Planas
Sep 24, 1999·Current Opinion in Nephrology and Hypertension·S L Ashworth, B A Molitoris
Apr 20, 2002·Circulation Research·Yaara Ben-YosefAriel Miller
May 9, 2002·American Journal of Physiology. Renal Physiology·Tokunori YamamotoMichael S Goligorsky
Aug 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ivan B LobovRichard A Lang
Mar 26, 2003·Journal of the American College of Cardiology·Hirofumi KasaharaHidezo Mori
Apr 23, 2003·The American Journal of Pathology·Sébastien Le JanStéphane Germain
Dec 20, 2003·The Biochemical Journal·Yu-Lin YangYu-Lun Huang
Jan 14, 2004·Kidney International·Christoph DanielChristian Hugo
Feb 28, 2004·Nephron. Experimental Nephrology·Kathrin HocheggerAlexander R Rosenkranz
Mar 11, 2004·Journal of Periodontal Research·Jeong Tae KohKyung Keun Kim
Jul 22, 2004·Contributions to Nephrology·Miet Schetz
Nov 24, 2004·Biochemical and Biophysical Research Communications·Koji IwanagaIssei Komuro

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Citations

Jan 26, 2013·Critical Care : the Official Journal of the Critical Care Forum·Catarina TeixeiraUNKNOWN NEFROlogia e Cura INTensiva (NEFROINT) investigators
Sep 17, 2011·Journal of the American Society of Nephrology : JASN·Gunnar SchleyCarsten Willam
Nov 22, 2011·Seminars in Nephrology·Sagar U Nigwekar, Sushrut S Waikar
Jul 15, 2015·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Jean-Paul DecuypereIna Jochmans
Mar 28, 2009·Critical Care Medicine·Brad E Cooper
Apr 7, 2007·Current Opinion in Anaesthesiology·Dean R Jones, H Thomas Lee
Feb 27, 2007·Molecular and Cellular Biochemistry·Natarajan AravindanAndrew D Shaw
Jul 26, 2015·Clinical Journal of the American Society of Nephrology : CJASN·Jay L Koyner
Apr 11, 2008·Critical Care Medicine·Sean M BagshawJohn A Kellum
Nov 8, 2016·Current Opinion in Nephrology and Hypertension·Jay L Koyner, Lakhmir S Chawla
Jul 31, 2019·Kidney & Blood Pressure Research·Daniel PatschanOliver Ritter

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