Effect of fusion protein cleavage site sequence on generation of a genotype VII Newcastle disease virus vaccine

PloS One
Vinoth K ManoharanSiba K Samal

Abstract

Newcastle disease (ND) causes severe economic loss to poultry industry worldwide. Frequent outbreaks of ND in commercial chickens vaccinated with live vaccines suggest a need to develop improved vaccines that are genetically matched against circulating Newcastle disease virus (NDV) strains. In this study, the fusion protein cleavage site (FPCS) sequence of NDV strain Banjarmasin/010 (Banj), a genotype VII NDV, was individually modified using primer mutagenesis to those of avian paramyxovirus (APMV) serotypes 2, 7 and 8 and compared with the recombinant Banjarmasin (rBanj) with avirulent NDV LaSota cleavage site (rBanj-LaSota). These FPCS mutations changed the in vitro cell-to-cell fusion activity and made rBanj FPCS mutant viruses highly attenuated in chickens. When chickens immunized with the rBanj FPCS mutant viruses and challenged with the virulent Banj, there was reduced challenge virus shedding observed compared to chickens immunized with the heterologous vaccine strain LaSota. Among the genotype VII NDV Banj vaccine candidates, rBanj-LaSota and rBanj containing FPCS of APMV-8 induced highest neutralizing antibody titers and protected chickens with reduced challenge virus shedding. These results show the effect of the F pr...Continue Reading

References

Aug 10, 2000·Revue Scientifique Et Technique·D J Alexander
Dec 4, 2003·Microbial Pathogenesis·Aruna PandaSiba K Samal
Dec 17, 2011·PloS One·Krishnaswamy G TirumurugaanSubbiah Elankumaran
Aug 16, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Diego G DielClaudio L Afonso
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Dec 17, 2014·Biologicals : Journal of the International Association of Biological Standardization·Stivalis Cardenas-GarciaClaudio L Afonso

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