PMID: 2112515Jun 15, 1990Paper

Effect of gamma interferon on HLA class-I and -II transcription and protein expression in human breast adenocarcinoma cell lines

International Journal of Cancer. Journal International Du Cancer
N Jabrane-FerratF Calvo

Abstract

The spontaneous expression of HLA class-I and class-II molecules in 5 human breast carcinoma cell lines, MCF-7, T47D, ZR75-1, HSL-53, MDA-MB 231, and their modulation during IFN-gamma treatment, are reported. The expression of cell-surface determinants was examined by indirect immunofluorescence using monoclonal antibodies (MAbs) specific for HLA class-I and class-II (DR, DQ and DP) antigens. The biosynthesis and maturation of these molecules were analyzed by 2-dimensional gel electrophoresis analysis (2D-PAGE) of class I, DR alpha, beta and invariant immunoprecipitates. Transcription was analyzed by Northern blot hybridization with HLA class-I and -II cDNA-specific probes. In all cell lines, more than 80% of cells expressed HLA class-I antigens at their surface. 2D-PAGE and mRNA studies showed a variable basal level of HLA class-I biosynthesis and transcription with a constant increase after 1,000 U/ml IFN-gamma treatment. HLA class-II determinants were totally absent from the surface of MCF-7, MDA MB231, ZR75-1 and T47D but they were detected in a small subpopulation of HSL-53 cells (DR 6%, DQ 6%, DP 20%). Spontaneous biosynthesis of HLA-DR molecules in immunoprecipitates analyzed by 2D-PAGE or transcripts in Northern blot we...Continue Reading

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Citations

Mar 31, 2000·Trends in Biotechnology·M XuR E Humphreys
Jun 8, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xue-Fan CuiKei Satoh
Jan 1, 1991·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·A PorgadorL Eisenbach
Oct 22, 2008·Experimental and Molecular Pathology·Sally A duPre'Kenneth W Hunter
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Sharon A OldfordSheila Drover
Aug 23, 2020·Journal of Clinical Medicine·Maria-Filothei LazaridouBarbara Seliger
Nov 23, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Margaret L AxelrodJustin M Balko
Sep 17, 2020·The Journal of Clinical Endocrinology and Metabolism·Jingtai ZhiMing Gao
Jul 16, 2021·Frontiers in Immunology·Elodie RenaudeOlivier Adotévi

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