Effect of gas phase on carbohydrate metabolism in Ascaris suum larvae

Molecular and Biochemical Parasitology
L Vanover-Dettling, P R Komuniecki

Abstract

The in vitro metabolism of [1-13C]glucose by Ascaris suum third and fourth-stage larvae was analyzed under different gas phases using 13C nuclear magnetic resonance spectroscopy (13C-NMR). Third-stage larvae (L3) incubated under a gas phase of 85% N2/5% O2/10% CO2 produced trace amounts of [13C]succinate, and molted to fourth-stage larvae (L4) between days 3 and 4 in vitro. However, they appeared to arrest as L3s when incubated under air, or 85% N2/5% O2/10% CO2 in the presence of 2 mM potassium cyanide, or 95% N2/5% CO2. Day 12 L4 (eight days after molting) incubated under 85% N2/5% O2/10% CO2, or 95% N2/5% CO2, or 94% N2/1% O2/5% CO2, produced succinate, acetate, propionate and the branched-chain fatty acids 2-methylvalerate and 2-methylbutyrate by fermentative pathways characteristic of adult body wall muscle. In contrast, when Day 12 L4 were incubated under air, only trace amounts of these acids were detected in the incubation medium. Thus, L4 are capable of synthesizing end-products typical of the adult even in the presence of oxygen, as long as the CO2 tensions are above 5%. As would be predicted, activities of enzymes involved in aerobic metabolism, including citrate synthase, isocitrate dehydrogenase, and cytochrome oxi...Continue Reading

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Citations

Jul 1, 1991·Trends in Pharmacological Sciences·C N Falany
Jul 1, 1992·Molecular and Biochemical Parasitology·S M SimsD P Thompson
Mar 1, 1992·Molecular and Biochemical Parasitology·K R JohnsonM J Wheelock
Feb 1, 1996·Molecular and Biochemical Parasitology·S TakamiyaK Kita
Jul 1, 1996·Molecular and Biochemical Parasitology·S TakamiyaT Aoki
Jan 1, 1994·Parasitology Today·A G Tielens
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A LesoonR Komuniecki
Feb 8, 1993·Biochimica Et Biophysica Acta·S TakamiyaT Aoki
Dec 27, 2007·Archives of Biochemistry and Biophysics·Muneaki HashimotoTakashi Aoki

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