Effect of genistein, a natural soy isoflavone, on pancreatic β-cells of streptozotocin-induced diabetic rats: Histological and immunohistochemical study

Journal of Microscopy and Ultrastructure
Eman Ali El-Kordy, Abdulrahman Mohammed Alshahrani

Abstract

Diabetes mellitus is one of the oldest disorders that is rapidly emerging as a global health problem. Soy genistein is a legume that has numerous health benefits. This work aimed to study the effect of different doses of genistein on histological, immunohistochemical and morphometrical changes in β-cells of streptozotocin (STZ)-induced diabetic rats and to correlate these effects with plasma glucose and insulin levels. Fifty adult male rats were divided into five equal groups. Group I served as a control. Group II received genistein. Group III comprised STZ-induced diabetic rats. Group IV diabetic animals treated with low dosage genistein. Group V diabetic animals treated with high dosage genistein. Genistein was given for 4 weeks after STZ injection. Rats were sacrificed and pancreatic specimens were taken for light and electron microscopic examination. Blood samples were collected for detection of serum glucose and insulin levels. After diabetic induction, the islets appeared shrunken with cytoplasmic vacuolation of their cells and negative insulin immunoreaction. Ultrastructurally, β-cells showed darkly stained nuclei with marked loss of granules. Morphometrically, significant loss of β-cells was detected. The serum insulin ...Continue Reading

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Citations

Feb 27, 2019·Pharmacological Reports : PR·Vinay Bharadwaj TatipamulaRajendra Prasad Yejella
Oct 28, 2019·Hormone Molecular Biology and Clinical Investigation·Rafighe GhiasiMohammad Reza Alipour
May 13, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Ahmet UyarZabit Yener
May 31, 2019·International Journal of Molecular Sciences·Adriano Costa de CamargoAndrés R Schwember
Dec 26, 2015·Nutrition & Metabolism·Ramachandran Vinayagam, Baojun Xu
Jan 1, 2021·International Journal of Molecular Sciences·Alina Kuryłowicz
Jan 16, 2021·Food & Nutrition Research·Rongrong LuZhuoqin Jiang
Jul 3, 2021·International Journal of Molecular Sciences·Shiyao ZhangSiyu Chen
Jul 13, 2021·Critical Reviews in Food Science and Nutrition·Saied A AboushanabElena G Kovaleva
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kanwal RehmanMuhammad Sajid Hamid Akash

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Methods Mentioned

BETA
light microscopy
transmission electron microscopy
ELISA

Software Mentioned

Excel
SPSS
Leica Qwin

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