PMID: 2492152Jan 1, 1989Paper

Effect of gentamicin on phospholipid metabolism in cultured rabbit proximal tubular cells

The American Journal of Physiology
L S RamsammyG J Kaloyanides

Abstract

We examined the hypothesis that the accumulation of phospholipid in cells exposed to gentamicin is due to impaired degradation. Experiments were performed in rabbit proximal tubular cells grown in primary culture. Cells exposed to 10(-3) M gentamicin manifested myeloid body formation and a progressive increase in total phospholipid that by day 6 was 44% higher than that of control cells and reflected increases of phosphatidylinositol of 235%, phosphatidylcholine of 60%, phosphatidylethanolamine of 90%, and phosphatidylserine of 55% above control values. Gentamicin impaired the degradation of these phospholipids. The t1/2 of the phospholipid pool labeled with [3H]myoinositol increased 146% from 1.17 (control) to 2.88 days (gentamicin); the t1/2 of the [3H]choline pool increased 34% from 1.77 to 2.38 days; the t1/2 of the [3H]ethanolamine pool increased 57% from 3.14 to 4.93 days; the t1/2 of the [3H] serine pool increased 37% from 6.30 to 8.63 days. Exposure of cells to gentamicin for 2 days also stimulated increased incorporation of [3H]myoinositol (68%) and [3H]ethanolamine (59%) into phospholipid. The data are consistent with the hypothesis that gentamicin inhibits the activity of lysosomal phospholipases that results in the ...Continue Reading

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Citations

Dec 1, 1994·Digestive Diseases and Sciences·H C Lin
Jan 23, 1998·JPEN. Journal of Parenteral and Enteral Nutrition·S KleinJ L Rombeau
Jun 1, 1995·Cytotechnology·I Kuhlmann
Mar 1, 2013·Cardiovascular Engineering and Technology·Aditya KumarK Jane Grande-Allen
Jun 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K G Dickman, J L Renfro
Jan 1, 1993·Archives of Toxicology·I OhnoO Sakai
Jul 30, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Takatoshi Karasawa, Peter S Steyger
Jan 3, 2001·Journal of the American Society of Nephrology : JASN·David P SundinBruce A Molitoris
Sep 1, 1990·Toxicology Letters·G GstraunthalerW Pfaller
Dec 1, 1990·Biochemical Pharmacology·G LaurentP M Tulkens
Sep 24, 2010·Kidney International·Jose M Lopez-NovoaFrancisco J Lopez-Hernandez

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