Effect of glucagon on digestive enzyme synthesis, transport and secretion in mouse pancreatic acinar cells

The Journal of Physiology
M Singh

Abstract

1. Effect of glucagon on amylase secretion and lactic dehydrogenase (LDH) release from functionally intact dissociated pancreatic acinar cells and acini was studied. 2. In dissociated rat pancreatic acinar cells, the rate of amylase secretion was increased by 70% with bethanechol (maximally effective concentration, 10(-4) M) and 125% with A23187 (10(-5) M), but the response to cholecystokinin-pancreozymin (CCK-PZ) was inconsistent. In dissociated cells from mouse pancreas, the increases amounted to 78% with bethanechol (10(-4) M), 134% with A23187 (10(-5) M) and 82% with CCK-PZ (maximally effective concentration, 0 . 01 u. ml.-1). Glucagon in concentrations ranging from 10(-7) to 10(-4) M increased amylase secretion by 3, 26, 67 and 80%, whereas secretin (10(-8)--10(-5) M) increased amylase secretion by 8, 39, 88 and 138%. LDH release was increased with A23187 in concentrations greater than 10(-6) M. 3. CCK-PZ, bethanechol and A23187 used in maximal concentrations potentiated the effect of a submaximal dose of glucagon whereas secretin did not have an additive or a potentiating effect. 4. Pancreatic acini were approximately 3 times more responsive to secretagogues than cells. The dose--response curves to bethanechol, glucagon a...Continue Reading

Citations

Jan 1, 1987·Digestive Diseases and Sciences·M Singh, M S Bandisode
May 31, 1985·Biochemical and Biophysical Research Communications·M S Bandisode, M Singh
Apr 17, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Savio G BarretoGino T P Saccone
Jan 1, 1986·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·X K Xiong
Jun 11, 1993·European Journal of Pharmacology·A HoriuchiS Chiba
Oct 1, 1994·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·J von SchönfeldM K Müller

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