Effect of glucocorticoid hormones on viral gene expression, growth, and dysplastic differentiation in HPV16-immortalized ectocervical cells

Experimental Cell Research
S KhareA Pater

Abstract

Steroid hormones are proposed to act as cofactors with human papillomaviruses (HPVs) in the etiology of cervical cancer. We and others reported that progesterone and glucocorticoid hormones induce the expression of HPV16 via three glucocorticoid response elements (GREs) in the viral regulatory region. Consensus GREs (GREcs) are useful in other systems for examining the effect of hormones after enhancing the response with mutated GREc constructs. Therefore, this study used human ectocervical cells (HEC) and HPV type 16 containing three GREcs to establish immortalized cells (HEC-16GREc). Northern blot assays showed that the level of viral E6-E7 oncogene RNA was increased by hormones substantially more in HEC-16GREc than in wild-type HPV16-immortalized human ectocervical cells (HEC-16). The saturation density and the hormone response of the growth rate were significantly higher for HEC-16GREc and the doubling was faster in the presence of hormone than for HEC-16. Although both were nontumorigenic, only HEC-16GREc showed anchorage-independent growth, which was dependent on hormone. Also, HEC-16GREc were more abnormal in their epithelium differentiation pattern in organotypic (raft) cultures. Furthermore, hormone-treated HEC-16GREc ...Continue Reading

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Citations

Jun 6, 2002·Journal of the European Academy of Dermatology and Venereology : JEADV·G von KroghS Syrjänen
Mar 10, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·C. J. KimJ. S. Park
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Aug 31, 2004·Pediatric Pulmonology·S L Lee, Y Hui
Jan 8, 2003·International Journal of Cancer. Journal International Du Cancer·Ethel-Michele de Villiers
Jun 16, 2000·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·E Armbruster-MoraesM Zugaib
May 14, 2011·Journal of Medical Virology·Yan Jun LeiPeter C Angeletti
Mar 24, 2005·The Journal of Biological Chemistry·Tzung-Chieh TsaiShow-Li Chen
May 19, 2001·The Journal of Biological Chemistry·L L CollinsC Chang
May 29, 2013·Transplant Infectious Disease : an Official Journal of the Transplantation Society·T SriP Stratton
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Nikita AggarwalAlok C Bharti

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