Effect of glucocorticoids on neonatal rabbit renal cortical sodium-inorganic phosphate messenger RNA and protein abundance

Pediatric Research
S PrabhuM Baum

Abstract

Administration of glucocorticoids to neonates increases proximal tubule volume absorption by increasing glucose, bicarbonate, and amino acid transport. We have recently demonstrated that glucocorticoids may contribute to the maturational decrease in phosphate transport. This study examines the maturation of NaPi-6 [the regulated proximal tubule sodium-inorganic phosphate (Na-Pi) transporter] mRNA and protein abundance and the mechanism for the decrease in phosphate transport by glucocorticoids. Weaned young rabbits (5 wk) had a 2-fold greater brush border membrane NaPi-6 protein abundance than that measured in adults. Renal cortical NaPi-6 mRNA abundance was comparable in neonates (less than 10 d of age) and adults. Renal brush border membrane vesicles from dexamethasone-treated neonatal rabbits (10 micrograms/100 g of body weight for 4 d) had a lower rate of Na-Pi transport than vehicle-treated controls (46.8 +/- 6.5 versus 71.0 +/- 9.0 pmol 32P/10 s/mg of protein, p < 0.05). Abundance of NaPi-6 protein in brush border membrane vesicles was 3-fold lower in newborn rabbits treated with pharmacologic doses of dexamethasone than in vehicle-treated controls. NaPi-6 mRNA abundance was the same in both groups. NaPi-1, a brush border...Continue Reading

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Citations

May 13, 2011·American Journal of Physiology. Renal Physiology·Jyothsna GattineniMichel Baum
Oct 4, 2000·Physiological Reviews·H MurerJ Biber
Sep 14, 2013·Physiological Reports·Catherine JosephMichel Baum
Nov 22, 2013·American Journal of Physiology. Renal Physiology·Jyothsna GattineniMichel Baum
Nov 21, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Jyothsna Gattineni, Michel Baum
Jan 14, 1999·The American Journal of Physiology·M ArarM Levi
Jan 22, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jaap MulderRaymond Quigley
Nov 24, 1999·The American Journal of Physiology·Y S GunerF K Ghishan
Mar 17, 2011·Advances in Chronic Kidney Disease·Ian ForsterAndreas Werner

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