PMID: 6337894Feb 1, 1983Paper

Effect of glucose stimulation on 45calcium uptake and total calcium content of pancreatic islets of fed and fasted rats and obese hyperglycemic mice

Diabetes
G H WoltersW Konijnendijk

Abstract

Effects of fasting on glucose-induced changes of various calcium parameters were investigated. The total calcium content of islets of fed rats and obese mice, determined by fluorometric microanalysis, amounted to 575 +/- 25 and 600 +/- 75 pmol/microgram DNA, respectively. Fasting for 24 or 72 h had no effect on the calcium content of rat islets, but increased that of mice by 30%. Glucose stimulation did not alter their total calcium content. The amount of 45Ca (as percentage of total islet calcium content) taken up over 30 min by fed mouse islets at 2.5, 10, and 15 mM glucose was equivalent to 32%, 41%, and 44% and by fed rat islets to 25%, 31%, and 34%, respectively. The net uptake of fed rat islets over 60 min at 2.5 and 15 mM glucose amounted to 36% and 61%. Fasting for 24 h inhibited insulin secretion over 30 min but not 45Ca uptake, whereas both parameters were inhibited after fasting for 72 h. Insulin secretion of mouse islets, was inhibited after 24 and 72 h of fasting, but 45Ca uptake was not significantly affected. Since glucose stimulates 45Ca uptake without changing the total islet calcium content, it must represent 45Ca- 40Ca exchange. Stimulation of insulin secretion of rat islets is associated with an increased ca...Continue Reading

Citations

Jul 10, 2003·Molecular and Cellular Endocrinology·Yingrao Tian, S G Laychock
Oct 1, 1991·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Y SarriR Gomis

Related Concepts

Calcium
Dose-Response Relationship, Drug
Fasting
Glucose, (beta-D)-Isomer
Hyperglycemia, Postprandial
Novolin
Islets of Langerhans
Obesity
August Rats

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