Effect of glutamate on lysosomal membrane permeabilization in primary cultured cortical neurons

Molecular Medicine Reports
Min YanWei Yin

Abstract

Glutamate is the principal neurotransmitter in the central nervous system. Glutamate-mediated excitotoxicity is the predominant cause of cerebral damage. Recent studies have shown that lysosomal membrane permeabilization (LMP) is involved in ischemia‑associated neuronal death in non‑human primates. This study was designed to investigate the effect of glutamate on lysosomal stability in primary cultured cortical neurons. Glutamate treatment for 30 min induced the permeabilization of lysosomal membranes as assessed by acridine orange redistribution and immunofluorescence of cathepsin B in the cytoplasm. Inhibition of glutamate excitotoxicity by the NMDA receptor antagonist MK‑801 and the calcium chelator ethylene glycol‑bis (2‑aminoethylether)‑N, N, N', N'‑tetraacetic acid, rescued lysosomes from permeabilization. The role of calpain and reactive oxygen species (ROS) in inducing LMP was also investigated. Ca2+ overload following glutamate treatment induced the activation of calpain and the production of ROS, which are two major contributors to neuronal death. It has been reported that lysosomal‑associated membrane protein 2 (LAMP2) and heat shock protein (HSP)70 are two calpain substrates that promote LMP in cancer cells; however...Continue Reading

References

Jan 1, 1990·Annual Review of Neuroscience·D W Choi, S M Rothman
Jan 1, 1990·Photochemistry and Photobiology·J M ZdolsekU T Brunk
Mar 24, 1995·The Journal of Biological Chemistry·S J MartinD R Green
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y LiD Schubert
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Tsuyoshi Nishi, Michael Forgac
Apr 15, 2003·Brain Research·Bipanjeet SekhonAvtar K Singh
Jan 16, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrey Y AbramovMichael R Duchen
Apr 13, 2004·Oncogene·Maria Eugenia GuicciardiGregory J Gores
Aug 10, 2004·The Journal of Experimental Biology·David S WarnerInes Batinić-Haberle
Aug 18, 2004·The Journal of Experimental Medicine·Jesper NylandstedMarja Jäättelä
Jul 12, 2005·Current Biology : CB·Popi SyntichakiNektarios Tavernarakis
Dec 17, 2008·Annals of the New York Academy of Sciences·Mark P Mattson
Oct 24, 2009·The Journal of Biological Chemistry·Chisato KubotaToshiyuki Takeuchi
Jan 16, 2010·Apoptosis : an International Journal on Programmed Cell Death·Ann-Charlotte JohanssonKarin Ollinger
Feb 23, 2010·Biochemical and Biophysical Research Communications·Shunya Sahara, Tetsumori Yamashima
Aug 6, 2010·Progress in Neurobiology·R Anne StetlerJun Chen
Jul 7, 2011·Mechanisms of Ageing and Development·Jenq-Lin YangVilhelm A Bohr
Nov 6, 2012·European Journal of Pharmacology·Ankita MehtaP L Sharma
Jun 12, 2013·Biochimica Et Biophysica Acta·Gloria E Villalpando Rodriguez, Alicia Torriglia

❮ Previous
Next ❯

Citations

Feb 7, 2018·Frontiers in Cellular Neuroscience·Shuchao WangKun Xiong
Mar 1, 2018·Physiological Reviews·Michael FrickerGuy C Brown
Aug 9, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Zheng-Yin PanLiang He
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daisuke HattaNobuhisa Iwata
Dec 15, 2020·Translational Neuroscience·Ranyah Shaker M LabbanAfaf El-Ansary
Apr 8, 2020·Free Radical Biology & Medicine·Pandian NagakannanEftekhar Eftekharpour

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
protein assay

Software Mentioned

SPSS

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.